Table S1. Distribution of spo0A, sspA and dpaAB genes and the ability to form spores within the representatives of Firmicutes with completely sequenced genomes.

Table S2. Taxonomy of Spo0A-encoding non-spore-forming Firmicutes.

Table S3. Distribution of known sporulation genes among 122 Spo0A-encoding firmicute genomes. A constantly updated version of this file is being maintained at the website

Table S4. Novel enzymatic function assignments for sporulation-related proteins.

Fig. S1. Correlation of spore formation and genome size within the representatives of class (A) Bacilli and (B) Clostridia.

Fig. S2. Comparison of the phylograms built from (A) concatenated sequences of 50 ribosomal proteins and (B) profiles of gene presence and absence from Table S3. Note the absence in (B) of the non-sporulating Macrococcus caseolyticus and Exiguobacterium spp. (red outline) and the shifted positions of Lysinibacillus sphaericus (green outline) and Alicyclobacillus acidocaldarius and Kyrpidia tusciae (blue outline).

Fig. S3. Amino acid substitutions in the active sites of peptidase M23-like (LytM) domains of SpoIIQ, CD0125 and SpoIVFA families.

Fig. S4. Sequence alignment of some known and newly translated SpoVM proteins.

emi2841_sm_FigureS1-S2-S3-S4.pdf136KSupporting info item
emi2841_sm_TableS1-S2-S4.pdf282KSupporting info item
emi2841_sm_TableS3.xlsx703KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.