Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses
Version of Record online: 14 AUG 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 409–418, February 2013
How to Cite
Wang, Z.-L., Zhang, L.-B., Ying, S.-H. and Feng, M.-G. (2013), Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses. Environmental Microbiology, 15: 409–418. doi: 10.1111/j.1462-2920.2012.02848.x
- Issue online: 28 JAN 2013
- Version of Record online: 14 AUG 2012
- Accepted manuscript online: 25 JUL 2012 03:52AM EST
- Received 4 June, 2012; revised 22 July, 2012; accepted 22 July, 2012.
Table S1. Paired primers designed for the manipulation of five catalase genes in B. bassiana (Bb2860).
Fig. S1. Disruption and complement of five catalase genes from B. bassiana (Bb2860). A. Diagram for the construct of a single-gene knockout. B−F. Identification of disrupted and rescued catA, catB, catP, catC and catD respectively. Reverse transcriptional PCR (lanes 1−3) and Southern blotting (lanes 4−6) were performed for detecting the presence or absence of each target gene in the cDNAs and genomic DNAs of knockout mutant (lanes 1 and 4), wild type (lanes 2 and 5) and complement mutant (lanes 3 and 6) respectively. The restriction enzymes used in the digestion of genomic DNAs for probing catA, catB, catP, catC and catD in Southern blotting were XbaI/NcoI, XbaI/BamHI, EcoRI, EcoRI/HindIII, BamHI/HindIII respectively.
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