The effect of zinc limitation on the transcriptome of Pseudomonas protegens Pf-5
Version of Record online: 19 AUG 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 702–715, March 2013
How to Cite
Lim, C. K., Hassan, K. A., Penesyan, A., Loper, J. E. and Paulsen, I. T. (2013), The effect of zinc limitation on the transcriptome of Pseudomonas protegens Pf-5. Environmental Microbiology, 15: 702–715. doi: 10.1111/j.1462-2920.2012.02849.x
- Issue online: 4 MAR 2013
- Version of Record online: 19 AUG 2012
- Accepted manuscript online: 27 JUL 2012 06:31AM EST
- Manuscript Accepted: 21 JUL 2012
- Manuscript Revised: 9 JUL 2012
- Manuscript Received: 4 APR 2012
- Macquarie University Research Excellence Scholarship (C.K.L.)
- Australian Research Council Discovery. Grant Number: DP110102680
Table S1. Genes significantly regulated by at least twofold by zinc limitation, as identified by SAM analysis of microarray data at a false discovery rate (FDR) of less than 5%.
Table S2. List of qRT-PCR primers utilized in this study.
Table S3. The putative Zur-binding motifs found using MAST version 4.8.1 (Bailey and Gribskov, 1998) with transcriptional fold changes of downstream genes, as determined from microarray data. The fold changes are reported in log2-based format.
Fig. S1. Role category analysis of microarray data. The numbers of upregulated and downregulated genes of Pf-5 grown in zinc-limited media versus zinc-replete media are respectively represented as blue and red bars. The genes are categorized according to 18 functional role categories (Hassan et al., 2010) and some genes can be placed in more than one category and thus counted more than once.
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