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Table S1. Genes significantly regulated by at least twofold by zinc limitation, as identified by SAM analysis of microarray data at a false discovery rate (FDR) of less than 5%.

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Table S2. List of qRT-PCR primers utilized in this study.

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Table S3. The putative Zur-binding motifs found using MAST version 4.8.1 (Bailey and Gribskov, 1998) with transcriptional fold changes of downstream genes, as determined from microarray data. The fold changes are reported in log2-based format.

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Fig. S1. Role category analysis of microarray data. The numbers of upregulated and downregulated genes of Pf-5 grown in zinc-limited media versus zinc-replete media are respectively represented as blue and red bars. The genes are categorized according to 18 functional role categories (Hassan et al., 2010) and some genes can be placed in more than one category and thus counted more than once.

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