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emi2855-sup-0001-suppl.doc85K

Supplementary materials and methods.

emi2855-sup-0002-fS1.docx43K

Fig. S1. Least significant intervals for all operational taxonomic units (OTUs) detected by the PhyloChip and nine bacterial classes shown to have significant treatment effects on richness (the number of OTUs) as determined by REML analysis.

emi2855-sup-0003-fS2.tif358K

Fig. S2. Mean gene copy number (± standard error) per nanogram of DNA for total bacteria and archaea, and the phyla Actinobacteria, Firmicutes and Bacteroidetes based on the 16S rRNA gene and total fungi based on the 18S rRNA gene assayed for 42 samples from ambient and elevated [CO2] (FACE) plots, with and without warming (+ 2°C) during 2007. Treatments codes are elevated [CO2] [control ambient CO2 or FACE (Free-Air Carbon dioxide Enrichment): C, F], warming (unwarmed or warmed: U, W) and plant functional type (non-Themeda or Themeda: NT, T). Samples were collected from two depths, upper at 0–5 cm and lower at 5–10 cm.

emi2855-sup-0004-tS1.docx14K

Table S1. Soil water potential (kPa) recorded for each ring in the 14 days prior to sampling TasFACE in 2007.

emi2855-sup-0005-tS2.pdf194K

Table S2. Distribution of OTUs detected in each treatment presented for classes with at least 10 OTUs detected in at least one treatment. Only OTUs present in at least two replicates were included.

emi2855-sup-0006-tS3.pdf120K

Table S3. Distribution of OTUs detected in each treatment presented for families with at least 10 OTUs detected in at least one treatment. Only OTUs present in at least two replicates of any treatment were included.

emi2855-sup-0007-tS4.pdf185K

Table S4. REML analysis of significant treatment effects on richness (the number of OTUs detected) for bacterial classes.

emi2855-sup-0008-tS5.docx14K

Table S5. Pearson and Kendall correlations for two ordination axes of non-metric dimensional scaling ordination and environmental variables (n = 42).

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