These authors contributed equally to this work.
Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation
Article first published online: 30 AUG 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 848–864, March 2013
How to Cite
Chen, Y., Yan, F., Chai, Y., Liu, H., Kolter, R., Losick, R. and Guo, J.-h. (2013), Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation. Environmental Microbiology, 15: 848–864. doi: 10.1111/j.1462-2920.2012.02860.x
- Issue published online: 4 MAR 2013
- Article first published online: 30 AUG 2012
- Accepted manuscript online: 3 AUG 2012 08:10AM EST
- Manuscript Accepted: 28 JUL 2012
- Manuscript Revised: 22 JUL 2012
- Manuscript Received: 28 MAY 2012
- National Natural Science Foundation of China. Grant Numbers: 31171809, 30971956
- Specialized Research Fund for the Doctoral Program of Higher Education of China. Grant Number: 20100097110010
- The Fundamental Research Funds for the Central Universities. Grant Number: KYZ201141
- China Scholarship Council. Grant Number: 2010685015
- Graduate Innovation Projects of Jiangsu Province. Grant Number: CX10B_3152
- NIH. Grant Numbers: GM18568, GM58213, GM82137
Fig. S1. Protection of tomato plants from bacterial wilt disease after treatment with wild type B. subtilis cells or various biofilm mutants. Shown in each panel, tomato plants were treated with one specific B. subtilis strain and 1 week later challenged with R. solanacearum. Thirty millilitres of the B. subtilis suspension (adjusted to a cell density of 108 cells per ml) was applied as irrigation to each pot. One week after inoculation with the B. subtilis suspension, 20 ml of R. solanacearum ZJ3721 cell suspension (~107 cells per ml) was drenched into each pot. Pictures were taken 4 weeks after treatment with B. subtilis cells. Bacillus subtilis strains applied in each panel (from A to I) are as follows: (A) wild type 3610; (B) ΔabrB; (C) ΔywcC; (D) ΔsinR; (E) ΔsinI; (F) ΔtasA; (G) ΔepsA-O; (H) ΔsrfAA; (I) H2O as a blank control.
Fig. S2. Biofilm colony morphology of the wild isolates CYBS-3, CYBS-7 and CYBS-16 on MSgg media. Cells were spotted on the solid MSgg plates and the plates were incubated for 72 h at 22°C before imaging. Scale bar, 0.2 cm.
Fig. S3. Antagonistic abilities of the B. subtilis wild isolates against various plant pathogens in vitro. Bacillus subtilis cells were inoculated as spots on the plates on which a lawn of cells for each tested plant pathogen were applied. Halos surrounding the B. subtilis inoculums indicated that the growth of pathogenic cells was inhibited by B. subtilis cells. Scale bars, 1.5 cm.
Table S1. Strains used in this study.
Table S2. Primers used in this study.
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