Fig. S1. Growth curves of P. putida KT2440G2 and F1G strains harbouring IncP-7 miniplasmids. The strains were grown in LB medium supplemented with Km and Gm as described in Experimental procedures and OD660 was measured at 30 min intervals using Bio-Photorecorder.


Fig. S2. Comparative genomic analysis between P. putida strains, KT2440 and F1. The colour scale for similarity values (%) is indicated at the bottom of picture. The PPGI-76 genes are indicated by cyan pentagons and the parI gene by orange one. The conserved genes between the two genomes are represented by pink pentagons while the cat operons are indicated by red pentagons. The insertion event of PPGI-76 into an ancestral KT2440 genome at the gene corresponding to Pput_2507 of F1 genome must have yielded PP3711 and PP3672 (blue pentagons) in the present form of KT2440 genome.


Fig. S3. Alignment of IncP-7 ParB proteins and PSPTOA0008 from pDC3000A by clustal w multiple alignment program ( Identical (*), strongly similar (:) and weakly similar (.) residues are shown below the sequences. Predicted secondary structure of ParBpDK1 is shown above. The N-terminal arginine residues conserved among the IncP-7 ParB proteins are highlighted in yellow box and the putative R-finger in red box. The different amino acid residue between ParBpDK1 and ParbpWW53 is boxed in cyan. The PSPTOA0008 protein showed 36% identity with ParBpDK1 while the cognate putative ParA protein, PSPTOA0009, showed 61% identity with ParApDK1. The parAB intergenic region on pDC3000A was found to contain similar 17 bp repeat sequences, 5′-TGaTGCTtGtAGCACCA-3′ and 5′-TGGTGCTaGtAGCACCA-3′.


Table S1. Primers used in this study.

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