Fig. S1. Schematic representation of the experimental tidal mesocosms as described in detail by Coulon and colleagues (2012).

A. Plan view showing the nine containers (32.5 × 42 × 25.5 cm) holding 20 sediment cores (9.8 cm high, 6.5 cm diameter) that were positioned above reservoir tanks. The three different treatments are indicated.

B. Side view showing the upper container holding the sediment cores and the lower reservoir, with the pump illustrating the flow of seawater.


Fig. S2. Solar irradiance experienced by the sediment during the course of the experiment. Two tube solar energy sensors (Solm) were placed at either end of the tanks used to hold the sediment cores (see Coulon et al., 2012 for details).


Fig. S3. Examples of fluorescence micrographs. Chlorophyll a fluorescence (F′; A, C, E, left column) and photosystem II operating efficiency (as measured by Fq/Fm′; B, D, F, right column), where green = least efficient, blue = intermediate, red = most efficient. A and B are from an oiled day-7 sediment core, Fq/Fm′ = 0.112. C and D are from an oiled day-14 sediment core, Fq/Fm′ = 0.172. E and F are from a non-oiled day-14 sediment core, Fq/Fm′ = 0.222. Each field of view is 640 × 480 μm.


Table S1. Primers used for amplification of nifH gene.

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