Present addresses: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK;
Impact of a simulated oil spill on benthic phototrophs and nitrogen-fixing bacteria in mudflat mesocosms
Article first published online: 16 SEP 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Baeza
Volume 15, Issue 1, pages 242–252, January 2013
How to Cite
Chronopoulou, P.-M., Fahy, A., Coulon, F., Païssé, S., Goñi-Urriza, M., Peperzak, L., Acuña Alvarez, L., McKew, B. A., Lawson, T., Timmis, K. N., Duran, R., Underwood, G. J. C. and McGenity, T. J. (2013), Impact of a simulated oil spill on benthic phototrophs and nitrogen-fixing bacteria in mudflat mesocosms. Environmental Microbiology, 15: 242–252. doi: 10.1111/j.1462-2920.2012.02864.x
- Issue published online: 3 JAN 2013
- Article first published online: 16 SEP 2012
- Accepted manuscript online: 8 AUG 2012 07:10AM EST
- Manuscript Accepted: 24 JUL 2012
- Manuscript Revised: 21 JUL 2012
- Manuscript Received: 27 APR 2012
- European Community. Grant Number: n°018391
- Greek State Scholarships Foundation
Fig. S1. Schematic representation of the experimental tidal mesocosms as described in detail by Coulon and colleagues (2012).
A. Plan view showing the nine containers (32.5 × 42 × 25.5 cm) holding 20 sediment cores (9.8 cm high, 6.5 cm diameter) that were positioned above reservoir tanks. The three different treatments are indicated.
B. Side view showing the upper container holding the sediment cores and the lower reservoir, with the pump illustrating the flow of seawater.
Fig. S2. Solar irradiance experienced by the sediment during the course of the experiment. Two tube solar energy sensors (Solm) were placed at either end of the tanks used to hold the sediment cores (see Coulon et al., 2012 for details).
Fig. S3. Examples of fluorescence micrographs. Chlorophyll a fluorescence (F′; A, C, E, left column) and photosystem II operating efficiency (as measured by Fq′/Fm′; B, D, F, right column), where green = least efficient, blue = intermediate, red = most efficient. A and B are from an oiled day-7 sediment core, Fq′/Fm′ = 0.112. C and D are from an oiled day-14 sediment core, Fq′/Fm′ = 0.172. E and F are from a non-oiled day-14 sediment core, Fq′/Fm′ = 0.222. Each field of view is 640 × 480 μm.
Table S1. Primers used for amplification of nifH gene.
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