Agrobacteria lacking ornithine lipids induce more rapid tumour formation
Article first published online: 9 SEP 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 895–906, March 2013
How to Cite
Vences-Guzmán, M. Á., Guan, Z., Bermúdez-Barrientos, J. R., Geiger, O. and Sohlenkamp, C. (2013), Agrobacteria lacking ornithine lipids induce more rapid tumour formation. Environmental Microbiology, 15: 895–906. doi: 10.1111/j.1462-2920.2012.02867.x
- Issue published online: 4 MAR 2013
- Article first published online: 9 SEP 2012
- Accepted manuscript online: 16 AUG 2012 01:47AM EST
- Manuscript Accepted: 4 AUG 2012
- Manuscript Revised: 2 AUG 2012
- Manuscript Received: 30 APR 2012
- CONACyT-Mexico. Grant Numbers: 46020-N, 153200
- DGAPA/UNAM. Grant Numbers: IN217907, IN201310
- LIPID MAPS. Grant Number: GM-069338
Fig. S1. Biosynthesis of OLs in Agrobacterium tumefaciens. The genes coding for OlsB and OlsA have been first identified in Sinorhizobium meliloti. Here we describe the identification of the gene encoding the OL hydroxylase OlsE introducing a hydroxyl group in the ornithine moiety of OL S1 leading to the formation of OL S2. LOL, lyso-ornithine lipid.
Fig. S2. Negative ion mode mass spectra of OL S1 (A) and OL S2 (B) purified from A. tumefaciens A208. The major OL [M-H]− ion species are labelled with arrows.
Fig. S3. Ornithine lipid S2 shows a different ninhydrin staining compared with OL S1. Unlabelled lipids were extracted according to Bligh and Dyer from 50 ml cultures of A. tumefaciens A208 (A) and R. tropici CIAT899 (B) grown in LB medium and separated in duplicate by two-dimensional TLC. Lipids were subjected to ninhydrin staining. The phospholipids PE, monomethyl PE (MMPE), and the OLs S1, S2, P1 and P2 are indicated.
Table S1. Bacterial strains and plasmids used in this study.
Table S2. Oligonucleotides used in this study. Introduced restriction sites are underlined.
Table S3. Sequences used for PSSM construction using the program Info-Gibbs. Sequences were from taken from Yuan and colleagues (2006).
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