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emi2868-sup-0001-si.ppt300K

Fig. S1. Maximum likelihood phylogenetic tree calculated for 16S rRNA gene sequences of Bacteroidetes and their closest relatives. Sequences obtained from Starnberger See (labelled Sta), Walchensee (Wal) and soil (JOCH, with A denoting antibiotic amendment in the culture), and on polypropylene (PP), glass (G) or steel (St). Numbers in the designations of sequence types refer to band numbering in Figs 1 and 2. Environmental sequences which were identical to those of cultured isolates are shaded in grey. 16S rRNA gene sequences of the Alphaproteobacteria Rhodopseudomonas palustris strain BisA53 and Caulobacter henricii subsp. aurantiacus strain ATCC15266 were used as outgroups.

emi2868-sup-0002-si.ppt467K

Fig. S2. Maximum likelihood phylogenetic tree calculated for 16S rRNA gene sequences of Alphaproteobacteria. For explanation of sequence labels compare legend with Fig. S1. 16S rRNA gene sequences of the Betaproteobacteria Pseudomonas saccharophila strain DSM 654T and Janthinobacterium lividum strain DSM1522T were used as outgroups.

emi2868-sup-0003-si.ppt234K

Fig. S3. Scheme for serial cultivation of freshwater microbial biofilms on solid substrates over four passages. Each passage was incubated for 4 weeks after which newly colonized solid substrate strips were transferred to new media containing a second sterile strip. In total four biofilms were generated from the surfaces incubated in freshwater enrichments and were subsequently analysed by DGGE fingerprinting. Incubation times for soil microbial biofilms were extended (see text).

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