Enrichment of previously uncultured bacteria from natural complex communities by adhesion to solid surfaces
Article first published online: 12 SEP 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 14, Issue 11, pages 2984–2997, November 2012
How to Cite
Gich, F., Janys, M. A., König, M. and Overmann, J. (2012), Enrichment of previously uncultured bacteria from natural complex communities by adhesion to solid surfaces. Environmental Microbiology, 14: 2984–2997. doi: 10.1111/j.1462-2920.2012.02868.x
- Issue published online: 24 OCT 2012
- Article first published online: 12 SEP 2012
- Accepted manuscript online: 16 AUG 2012 01:58AM EST
- Manuscript Accepted: 6 AUG 2012
- Manuscript Revised: 2 AUG 2012
- Manuscript Received: 31 MAY 2012
- BMBF (Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie). Grant Numbers: BIOLOG/01LC0021, 01LC0021A, 01LC0621C
Fig. S1. Maximum likelihood phylogenetic tree calculated for 16S rRNA gene sequences of Bacteroidetes and their closest relatives. Sequences obtained from Starnberger See (labelled Sta), Walchensee (Wal) and soil (JOCH, with A denoting antibiotic amendment in the culture), and on polypropylene (PP), glass (G) or steel (St). Numbers in the designations of sequence types refer to band numbering in Figs 1 and 2. Environmental sequences which were identical to those of cultured isolates are shaded in grey. 16S rRNA gene sequences of the Alphaproteobacteria Rhodopseudomonas palustris strain BisA53 and Caulobacter henricii subsp. aurantiacus strain ATCC15266 were used as outgroups.
Fig. S2. Maximum likelihood phylogenetic tree calculated for 16S rRNA gene sequences of Alphaproteobacteria. For explanation of sequence labels compare legend with Fig. S1. 16S rRNA gene sequences of the Betaproteobacteria Pseudomonas saccharophila strain DSM 654T and Janthinobacterium lividum strain DSM1522T were used as outgroups.
Fig. S3. Scheme for serial cultivation of freshwater microbial biofilms on solid substrates over four passages. Each passage was incubated for 4 weeks after which newly colonized solid substrate strips were transferred to new media containing a second sterile strip. In total four biofilms were generated from the surfaces incubated in freshwater enrichments and were subsequently analysed by DGGE fingerprinting. Incubation times for soil microbial biofilms were extended (see text).
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.