These authors contributed equally to this article.
Differentiated functions of Ras1 and Ras2 proteins in regulating the germination, growth, conidiation, multi-stress tolerance and virulence of Beauveria bassiana
Version of Record online: 7 SEP 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 447–462, February 2013
How to Cite
Xie, X.-Q., Guan, Y., Ying, S.-H. and Feng, M.-G. (2013), Differentiated functions of Ras1 and Ras2 proteins in regulating the germination, growth, conidiation, multi-stress tolerance and virulence of Beauveria bassiana. Environmental Microbiology, 15: 447–462. doi: 10.1111/j.1462-2920.2012.02871.x
- Issue online: 28 JAN 2013
- Version of Record online: 7 SEP 2012
- Accepted manuscript online: 16 AUG 2012 10:02PM EST
- Manuscript Revised: 8 AUG 2012
- Manuscript Accepted: 8 AUG 2012
- Manuscript Received: 22 JUL 2012
- Natural Science Foundation of China. Grant Numbers: 30930018, 31101489, 31021003
- Ministry of Science and Technology. Grant Numbers: 2011AA10A204, 2009CB118904
Table S1. The primers used for the manipulation of Ras1 and Ras2 genes in B. bassiana.
Table S2. Primers used to assess the transcript levels of downstream effector genes in the Ras1 and Ras2 mutants of B. bassiana via qRT-PCR.
Fig. S1. Construction and identification of B. bassiana Ras1 and Ras2 mutants.
A. Diagram for Ras2 knockout (see Table S1 for the used primers).
B. PCR detection for the disrupted and complemented Ras2 in the genomic DNAs of wild type (lanes 1 and 4), ΔRas2 (lanes 2 and 5) and ΔRas2/Ras2 (lanes 3 and 6) with paired primers R2id-F/R (lanes 1−3) and Sur2-F/R (lanes 4−6).
C. Southern blotting of Ras2 in the XbaI-digested DNA extracts from wild type (lane 1), ΔRas2 (lanes 2) and ΔRas2/Ras2 (lanes 3) using the probe amplified with R2S-F/R.
D and E. PCR detection for the presence of Ras1 (with partial PgpdA) and sur in the 3-day SDAY colonies of wild type (lane 1), mRas1G19V (lanes 2−4), mRas1D126A (lanes 5−7), mRas1 (lanes 8−10), ΔRas2/Ras1G19V (lanes 11−13), ΔRas2/Ras1D126A (lanes 14−16) and ΔRas2/Ras1 (lanes 17−19) respectively.
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