Research Article

Oral insecticidal activity of plant-associated pseudomonads

  1. Beat Ruffner1,
  2. Maria Péchy-Tarr2,
  3. Florian Ryffel1,
  4. Patrik Hoegger3,
  5. Christian Obrist3,
  6. Alfred Rindlisbacher3,
  7. Christoph Keel2,*,
  8. Monika Maurhofer1,*

Article first published online: 3 OCT 2012

DOI: 10.1111/j.1462-2920.2012.02884.x

Issue

Environmental Microbiology

Environmental Microbiology

Special Issue: Plant–Microbe Interactions

Volume 15, Issue 3, pages 751–763, March 2013

Additional Information

How to Cite

Ruffner, B., Péchy-Tarr, M., Ryffel, F., Hoegger, P., Obrist, C., Rindlisbacher, A., Keel, C. and Maurhofer, M. (2013), Oral insecticidal activity of plant-associated pseudomonads. Environmental Microbiology, 15: 751–763. doi: 10.1111/j.1462-2920.2012.02884.x

Author Information

  1. 1

    Plant Pathology, Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland

  2. 2

    Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland

  3. 3

    Syngenta Crop Protection AG, Stein, Switzerland

*For correspondence. E-mail monika.maurhofer@usys.ethz.ch; Tel. (+41) 44 632 38 69; Fax (+41) 44 632 15 72; E-mail christoph.keel@unil.ch; Tel. (+41) 21 692 56 36; Fax (+41) 21 692 56 05.

Publication History

  1. Issue published online: 4 MAR 2013
  2. Article first published online: 3 OCT 2012
  3. Accepted manuscript online: 4 SEP 2012 10:53PM EST
  4. Manuscript Accepted: 28 AUG 2012
  5. Manuscript Revised: 16 AUG 2012
  6. Manuscript Received: 17 JAN 2012

Funded by

  • Swiss National Foundation for Scientific Research. Grant Number: 3100A0-105881
  • Swiss State Secretariat for Education and Research. Grant Number: C07.0026

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Fig. S1. Development of S. littoralis larvae upon feeding on Fit toxin expressing E. coli. The larval development was recorded 8 days after exposure to E. coli BL21 overexpressing the FitD toxin. The percentage of survival rates (A), or the average weight (B) were determined on a total of 50 Spodoptera larvae. Based on multiple comparisons by Tukey HSD, no significant differences (P ≤ 0.05) could be detected among the empty vector carrying and the Fit toxin expressing E. coli strain. The horizontal bars indicate the standard errors of means.

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Table S1. Oligonucleotides used for Fit locus identification of Pseudomonas chlororaphis PCL1391.

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