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emi2894-sup-0001-si.pdf329K

Fig. S1. Concentrations of ammonium (•) and nitrite (□) in the preculture of Nitrosopumilus maritimus.

emi2894-sup-0001-si.pdf329K

Fig. S2. Gene expression of ‘Ca. Scalindua profunda’ ananmmox bacteria under anoxic versus oxic condition. RPKM = number of reads per kb of transcript per million mapped reads.

emi2894-sup-0001-si.pdf329K

Fig. S3. Base peak chromatogram of LC/MS analysis of GDGTs in the bioreactor. Concentrations of specific GDGTs and their structures are indicated.

emi2894-sup-0001-si.pdf329K

Fig. S4. Gene expression of selected Nitrosopumilus maritimus SCM1 genes under oxygen limitation.

emi2894-sup-0001-si.pdf329K

Fig. S5. Potential aerobic ammonia-oxidizing activity of the Nitrosopumilus maritimus culture (A) and Nitrosomonas-like AOB enrichment (B). Open squares (□) and closed squares (▪) indicate AOB enrichment with and without PTIO addition respectively.

emi2894-sup-0002-si.docx17K

Table S1. Primers for PCR amplification and sequencing.

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