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emi2895-sup-0001-si.avi2638K

emi2895-sup-0002-si.avi2889K

emi2895-sup-0003-si.avi3449K

Fig. S1. Video clips of biofilm structures formed by Hbt. salinarum DSM 3754T, R1 and t-ADL isolate DL24 on glass. Cells were incubated at 42°C for 14 days (Hbt. salinarum DSM 3754T and R1) or at 28°C (t-ADL isolate DL24) and then stained with acridine orange. Confocal scanning micrographs (CLSM) image data were processed using the ImageJ 3D viewer software.

emi2895-sup-0004-si.tif791K

Fig. S2. Cellular vitality of t-ADL DL24, Hfx. volcanii DSM 3757T and Hrr. lacusprofundi DL28 after 31–48 days of cultivation. Adherent cells were treated with propidium iodide (PI) according to the procedure of the fluorescence-based LIVE/DEAD assay. Overlay of differential interference contrast (DIC) microscopy and PI-staining visualizes the localization of dead cells (red).

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