Biofilm formation by haloarchaea
Article first published online: 12 OCT 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 14, Issue 12, pages 3159–3174, December 2012
How to Cite
Fröls, S., Dyall-Smith, M. and Pfeifer, F. (2012), Biofilm formation by haloarchaea. Environmental Microbiology, 14: 3159–3174. doi: 10.1111/j.1462-2920.2012.02895.x
- Issue published online: 4 DEC 2012
- Article first published online: 12 OCT 2012
- Accepted manuscript online: 19 SEP 2012 06:51AM EST
- Manuscript Accepted: 28 AUG 2012
- Manuscript Revised: 10 AUG 2012
- Manuscript Received: 8 FEB 2012
- Max Planck Society
Fig. S1. Video clips of biofilm structures formed by Hbt. salinarum DSM 3754T, R1 and t-ADL isolate DL24 on glass. Cells were incubated at 42°C for 14 days (Hbt. salinarum DSM 3754T and R1) or at 28°C (t-ADL isolate DL24) and then stained with acridine orange. Confocal scanning micrographs (CLSM) image data were processed using the ImageJ 3D viewer software.
Fig. S2. Cellular vitality of t-ADL DL24, Hfx. volcanii DSM 3757T and Hrr. lacusprofundi DL28 after 31–48 days of cultivation. Adherent cells were treated with propidium iodide (PI) according to the procedure of the fluorescence-based LIVE/DEAD assay. Overlay of differential interference contrast (DIC) microscopy and PI-staining visualizes the localization of dead cells (red).
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.