Technoreview: In vivo bioluminescence imaging for integrated studies of infection
Article first published online: 23 FEB 2004
Volume 6, Issue 4, pages 303–317, April 2004
How to Cite
Doyle, T. C., Burns, S. M. and Contag, C. H. (2004), Technoreview: In vivo bioluminescence imaging for integrated studies of infection. Cellular Microbiology, 6: 303–317. doi: 10.1111/j.1462-5822.2004.00378.x
- Issue published online: 23 FEB 2004
- Article first published online: 23 FEB 2004
- Received 5 November, 2003; revised 16 December, 2003; accepted 18 December, 2003.
Understanding biological processes in the context of intact organ systems with fine temporal resolution has required the development of imaging strategies that reveal cellular and molecular changes in the living body. Reporter genes that confer optical signatures on a given biological process have been used widely in cell biology and have been used more recently to interrogate biological processes in living animal models of human biology and disease. The use of internal biological sources of light, luciferases, to tag cells, pathogens, and genes has proved to be a versatile tool to provide in vivo indicators that can be detected externally. The application of this technology to the study of animal models of infectious disease has not only provided insights into disease processes, but has also revealed new mechanisms by which pathogens may avoid host defences during infection.