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Candida albicans and Streptococcus salivarius modulate IL-6, IL-8, and TNF-α expression and secretion by engineered human oral mucosa cells
Article first published online: 29 JUN 2004
DOI: 10.1111/j.1462-5822.2004.00420.x
1462-5822/asset/cover.gif?v=1&s=e30d0f6619b5d82d5a32c3d370df0428348dde7d "Cellular Microbiology")
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How to Cite
Mostefaoui, Y., Bart, C., Frenette, M. and Rouabhia, M. (2004), Candida albicans and Streptococcus salivarius modulate IL-6, IL-8, and TNF-α expression and secretion by engineered human oral mucosa cells. Cellular Microbiology, 6: 1085–1096. doi: 10.1111/j.1462-5822.2004.00420.x
Publication History
- Issue published online: 29 JUN 2004
- Article first published online: 29 JUN 2004
- Received 10 March, 2004; revised 27 April, 2004; accepted 3 May, 2004.
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Summary
We investigated the involvement of oral epithelial cells via two cytokines (IL-6 and TNF-α) and one chemokine (IL-8) in local defences against live yeast (Candida albicans) and bacteria (Streptococcus salivarius) using an engineered human oral mucosa model. We report that the yeast changed from the blastospore to the hyphal form and induced significant tissue disorganization at later contact periods (24 and 48 h) compared to the bacteria. However, this effect did not reduce the viability or total number of epithelial cells. Gene activation analyses revealed that IL-6, IL-8 and TNF-α mRNA levels rose in tissues in contact with live C. albicans or S. salivarius. Gene activation was followed by an upregulation of protein secretion. IL-6 levels were higher after contact with C. albicans than with S. salivarius. IL-8 levels after contact with S. salivarius were higher than with C. albicans. Our study suggests that S. salivarius is more efficient at inducing proinflammatory mediator release than C. albicans. These results provide additional evidence for the contribution of oral epithelial cells to the inflammatory response against fungi and bacteria.