Plant-induced cell death in the oomycete pathogen Phytophthora parasitica

Authors

  • Eric Galiana,

    Corresponding author
    1. Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique/Université Nice Sophia-Antipolis/Centre National de la Recherche Scientifique 400 Route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
    Search for more papers by this author
  • Marie-Pierre Rivière,

    1. Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique/Université Nice Sophia-Antipolis/Centre National de la Recherche Scientifique 400 Route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
    Search for more papers by this author
  • Sophie Pagnotta,

    1. Centre Commun de Microscopie Appliquée, Université de Nice-Sophia-Antipolis, Parc Valrose, 06103 Nice Cedex 2, France.
    Search for more papers by this author
  • Emmanuel Baudouin,

    1. Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique/Université Nice Sophia-Antipolis/Centre National de la Recherche Scientifique 400 Route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
    Search for more papers by this author
  • Franck Panabières,

    1. Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique/Université Nice Sophia-Antipolis/Centre National de la Recherche Scientifique 400 Route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
    Search for more papers by this author
  • Pierre Gounon,

    1. Centre Commun de Microscopie Appliquée, Université de Nice-Sophia-Antipolis, Parc Valrose, 06103 Nice Cedex 2, France.
    Search for more papers by this author
  • Laure Boudier

    1. Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique/Université Nice Sophia-Antipolis/Centre National de la Recherche Scientifique 400 Route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
    Search for more papers by this author

E-mail: galiana@antibes.inra.fr; Tel. (+33) 492 38 64 72; Fax (+33) 492 38 65 87.

Summary

The activation of programmed cell death in the host during plant–pathogen interactions is an important component of the plant disease resistance mechanism. In this study we show that activation of programmed cell death in microorganisms also regulates plant–pathogen interactions. We found that a form of vacuolar cell death is induced in the oomycete Phytophthora parasitica– the agent that causes black shank disease in Nicotiana tabacum– by extracellular stimuli from resistant tobacco. The single-celled zoospores underwent cell death characterized by dynamic membrane rearrangements, cell shrinkage, formation of numerous large vacuoles in the cytoplasm and degradation of cytoplasmic components before plasma membrane disruption. Phytophthora cell death required protein synthesis but not caspase activation, and was associated with the production of intracellular reactive oxygen species. This characterization of plant-mediated cell death signalling in pathogens will enhance our understanding of the biological processes regulating plant–pathogen interactions, and improve our ability to control crop diseases.

Ancillary