Present address: Division of Biology, Kansas State University, Manhattan, KS 66502, USA.
The parasite invasion marker SRPN6 reduces sporozoite numbers in salivary glands of Anopheles gambiae
Article first published online: 14 NOV 2007
© 2007 The Authors
Volume 10, Issue 4, pages 891–898, April 2008
How to Cite
Pinto, S. B., Kafatos, F. C. and Michel, K. (2008), The parasite invasion marker SRPN6 reduces sporozoite numbers in salivary glands of Anopheles gambiae. Cellular Microbiology, 10: 891–898. doi: 10.1111/j.1462-5822.2007.01091.x
- Issue published online: 6 DEC 2007
- Article first published online: 14 NOV 2007
- Received 27 June, 2007; revised 30 October, 2007; accepted 1 November, 2007.
For malaria transmission to occur, Plasmodium sporozoites must infect the salivary glands of their mosquito vectors. This study reports that Anopheles gambiae SRPN6 participates in a local salivary gland epithelial response against the rodent malaria parasite, Plasmodium berghei. We showed previously that SRPN6, an immune inducible midgut invasion marker, influences ookinete development. Here we report that SRPN6 is also specifically induced in salivary glands with the onset of sporozoite invasion. The protein is located in the basal region of epithelial cells in proximity to invading sporozoites. Knockdown of SRPN6 during the late phase of sporogony by RNAi has no effect on oocyst rupture but significantly increases the number of sporozoites present in salivary glands. Despite several differences between the passage of Plasmodium through the midgut and the salivary glands, this study identifies a striking overlap in the molecular responses of these two epithelia to parasite invasion.