Chlamydiae are Gram-negative obligate intracellular bacteria that cause diseases with significant medical and economic impact. Chlamydia trachomatis replicates within a vacuole termed an inclusion, which is extensively modified by the insertion of a number of bacterial effector proteins known as inclusion membrane proteins (Incs). Once modified, the inclusion is trafficked in a dynein-dependent manner to the microtubule-organizing centre (MTOC), where it associates with host centrosomes. Here we describe a novel structure on the inclusion membrane comprised of both host and bacterial proteins. Members of the Src family of kinases are recruited to the chlamydial inclusion in an active form. These kinases display a distinct, localized punctate microdomain-like staining pattern on the inclusion membrane that colocalizes with four chlamydial inclusion membrane proteins (Incs) and is enriched in cholesterol. Biochemical studies show that at least two of these Incs stably interact with one another. Furthermore, host centrosomes associate with these microdomain proteins in C. trachomatis-infected cells and in uninfected cells exogenously expressing one of the chlamydial effectors. Together, the data suggest that a specific structure on the C. trachomatis inclusion membrane may be responsible for the known interactions of chlamydiae with the microtubule network and resultant effects on centrosome stability.
Chlamydia trachomatis is a Gram-negative obligate intracellular bacterium responsible for a number of significant human diseases. C. trachomatis is comprised of over 15 unique serovars, among which are the etiological agents of trachoma, the leading cause of infectious blindness worldwide, while other serovars are the most common cause of sexually transmitted diseases (Schachter, 1999).
Given their localization within the inclusion membrane and access to the host cell cytoplasm, Incs are a logical choice for putative effectors responsible for mediating microtubule/centrosomal interaction. Here we describe microdomains present in the inclusion membrane that are comprised of host cell kinases and at least four Incs. Furthermore, we present data implicating these microdomains in interactions of the chlamydial inclusion with the microtubule network and centrosomes.
Src family kinases are recruited to the chlamydial inclusion
The Src family kinases are non-receptor membrane-associated tyrosine kinases that are involved in a number of cellular signalling pathways regulating such diverse functions as differentiation, motility, adhesion, apoptosis and membrane trafficking. Src, Fyn and Yes are ubiquitously expressed in mammalian cells and some functional redundancy is present, while the other family members are expressed in more specialized cells (Stein et al., 1994; Roche et al., 1995; Thomas and Brugge, 1997; Sandilands and Frame, 2008). Src family kinases are regulated by the phosphorylation state of multiple tyrosines within each kinase. Phosphorylation at Tyr216 and Tyr419 of human Src appears to be necessary for upregulated kinase activity, with autophosphorylation at 419 thought to be the predominant activation site. Conversely, phosphorylation at Tyr530 results in downregulated kinase activity (Roskoski, 2005). We examined the recruitment of the ubiquitously expressed Src family kinases (Src, Yes, Fyn) to the mature chlamydial inclusion. Fyn and Src, but not Yes, were detected in association with the C. trachomatis serovar L2 inclusion membrane (Fig. 1 and data not shown). When antibodies that recognize all forms of individual kinases regardless of activation state are used, the staining typically appears as a diffuse pattern around the periphery of the inclusion. However, when an antibody specific for phosphorylated Tyr419, which only recognizes enzymatically active forms of Src family kinases, was used, a defined punctate staining pattern was observed that when viewed tangentially appeared as discrete bar-like microdomains on the inclusion membrane (Fig. 1). Immunostaining followed by electron tomography was used to confirm the microdomain-like localization of active kinase at the C. trachomatis serovar L2 inclusion membrane. A single slice from electron tomographs of active kinase-labelled samples (Fig. 1) shows electron-dense diaminobenzidine (DAB) reaction product localized in microdomains at the inclusion membrane. Negative controls lacking active kinase antibody show no such labelling at the inclusion membrane. Typically, one to three microdomains were observed on each inclusion.
To further confirm this microdomain-like localization at the inclusion membrane an antibody targeting the secondary phospho-activation site (Tyr216) was used to label C. trachomatis L2-infected cells and showed an identical microdomain-like staining pattern as anti-Tyr419 (data not shown). This morphology suggests that the active Src family kinases are localized to specific microdomains within the inclusion membrane.
Because the Tyr419 activation site is conserved among Src, Yes and Fyn, the anti-active kinase antibody could potentially recognize the active form of any of these Src family kinases. Therefore, we used siRNA depletion of the three ubiquitously expressed kinases to unambiguously determine which were recruited to the chlamydial inclusion microdomains (Fig. 2). When the kinases were knocked down individually and compared with a non-targeting siRNA, depletion of Fyn had the most significant effect on active kinase recruitment to the inclusion membrane, although faint staining of some inclusions was still observed. Src depletion had a lesser effect and Yes had no observable effect. Additionally, simultaneous depletion of both Src and Fyn eliminated active kinase staining at the chlamydial inclusion indicating that either kinase may be recruited and thus potentially provide functional redundancy. No significant effect on inclusion development was observed in the double kinase knockdown (data not shown).
Active kinase recruitment is not conserved in all species of chlamydiae
We examined the inclusions of other chlamydial species and serovars to investigate whether active kinase recruitment represents a conserved process. Active kinase colocalized with the C. trachomatis serovar D inclusion membranes in a similar staining pattern as that observed for serovar L2 (Fig. 3), suggesting that active kinase recruitment may be conserved in the C. trachomatis LGV and oculo-urogenital biovars. HeLa monolayers were also infected with Chlamydia muridarum, C. caviae or C. pneumoniae and stained with active kinase antibody. Punctate staining was observed in association with the C. pneumoniae inclusion membrane, but not with C. muridarum or C. caviae inclusion membranes. Therefore, Src family kinase recruitment is not conserved in all species of chlamydiae.
Specific Incs colocalize with active kinase
Incs are expressed at the interface of the inclusion with the host cell cytosol (Rockey et al., 1995; Bannantine et al., 1998; 2000; Hackstadt et al., 1999) and are thus positioned to specifically regulate the interactions of the inclusion with the host cell. We therefore explored the possibility of Inc proteins being involved in the structure or function of the observed inclusion membrane microdomains. Antibodies against a panel Incs (Shaw et al., 2000) were screened for colocalization with active kinase in C. trachomatis L2-infected cells (Fig. 4). As previously described (Scidmore-Carlson et al., 1999), IncG displayed a uniform staining pattern around the periphery of the inclusion membrane. There is some overlap with active kinase but no apparent enrichment in association with the active kinase. However, four Incs (IncB, Inc101, Inc222 and Inc850) showed a discrete punctate microdomain-like staining pattern that colocalized with the active kinase. These Inc/active kinase microdomains varied in size and number but this variability did not correlate with multiplicity of infection (moi) or time post infection (data not shown). With few exceptions, the Inc protein overlap with active kinase is complete, in that microdomains contain both Incs and active kinase and both classes of protein are present along the entirety of the microdomain.
IncB, 101, 222 and 850 colocalize with each another
Each member of this subset of four Incs colocalized individually with active kinase. The observation that the individual Inc's colocalization with active kinase was virtually complete suggests that they exist in common microdomains. To confirm this hypothesis, Inc antibodies were directly conjugated to complementary Alexa Fluor dyes and pairs of Incs were observed for colocalization on the inclusion membrane. When IncB was used as the common Inc, Incs 101, 222 and 850 colocalized within IncB microdomains (Fig. 5). Identical results were obtained when Inc222 was used as the common antibody (data not shown).
Stable interaction of two of the microdomain Incs
We investigated the possibility of any direct interaction of the four microdomain Incs with each other. Co-immunoprecipitation experiments were performed using an antibody to each individual Inc followed by immunoblotting with antibodies to all Incs of this subclass (Fig. 6). An anti-rickettsia antibody was used as a negative control. Zwittergent 3-14 was used to solubilize the infected cells as it has previously been used to successfully preserve the interaction between an Inc and a host protein (Scidmore and Hackstadt, 2001). Western blots probed with Inc222 confirmed that Inc222 was pulled down using an Inc222 antibody (Fig. 6, lane 7). Inc222was also pulled down when an Inc850 antibody was used for the immunoprecipitation (Fig. 6, lane 9). Inc222 was not precipitated from uninfected lysate or when anti-rickettsia, anti-IncB or anti-Inc101 antibodies were used for the immunoprecipitations. Conversely, Incs B and 101 were only observed in lanes where the corresponding antibody was used for the immunoprecipitation (Fig. 6, lanes 3 and 5) and was not immunoprecipitated with antibodies to any other Inc proteins or the negative control. These results suggest that Inc222 and Inc850 physically interact with each other individually or as a complex but not with IncB or Inc101.
Inc microdomains are enriched in cholesterol
The chlamydial inclusion membrane contains cholesterol derived from the host cell (Carabeo et al., 2003). We questioned whether these localized microdomains on the inclusion membrane might have some altered lipid composition related to the recruitment of active Src family kinases and specific chlamydial inclusion membrane proteins. We therefore examined Inc microdomains for the presence of cholesterol. Fluorescent microscopy using filipin as a cholesterol probe showed an enrichment of cholesterol at the microdomains within the inclusion membrane (Fig. 7). These results are consistent with a unique structure and composition of the observed microdomains.
Inc microdomains associate with centrosomes
In order to elucidate possible functions of the Inc microdomains, a panel of antibodies against various cellular organelles or cytoskeletal structures was examined by fluorescence microscopy for association with the Inc microdomains. Only one antibody, to β-tubulin, appeared to indicate an interaction with Inc microdomains. It is known that chlamydial inclusions use interactions with the minus-end microtubule motor complex dynein to traffic to the MTOC where they associate with centrosomes (Grieshaber et al., 2006). Fluorescent microscopy revealed that Inc microdomains were consistently associated with the mitotic spindle pole in cells undergoing mitosis. When a section of the inclusion is viewed tangentially (Fig. 8), Inc microdomains appear as well-defined narrow bar-like structures on the inclusion membrane and the mitotic spindle appears to terminate at the Inc microdomain. During mitosis, one or both spindle poles may be observed in association with Inc microdomains.
Centrosomes are located at the convergence of the mitotic spindle apparatus; therefore, we labelled infected cells with γ-tubulin, which preferentially stains the centrosome. Centrosomes are observed in close apposition to the inclusion microdomains in both interphase and metaphase cells (Fig. 8), indicating that this association is not limited to mitosis. Similar to what is observed in β-tubulin-stained cells, one or both centrosomes can be associated with Inc microdomains.
Dynein plays a role in multiple cellular functions including organelle positioning and vesicular transport. It is also implicated in mitosis with a role in spindle organization and chromosome movement (Vallee and Sheetz, 1996; Karki and Holzbaur, 1999; Dujardin and Vallee, 2002). Consistent with this role, dynein is observed localized at kinetochores and spindle fibres with a focus at the spindle poles and is enriched at centrosomes during the S and G2 phases of the cell cycle (Quintyne and Schroer, 2002). Because of its role in trafficking nascent chlamydial inclusions to the MTOC and maintaining a tight interaction with centrosomes (Grieshaber et al., 2006), we examined the association of dynein with chlamydial inclusion microdomains (Fig. 8). Again, dynein was localized to mitotic spindles and appeared to focus at the Inc microdomain. During mitosis, one or both spindle poles could be observed in association with Inc microdomains. In non-mitotic cells, dynein was observed less frequently in association with microdomains although this likely reflects cell cycle dependent association of dynein with centrosomes (Quintyne and Schroer, 2002).
Ectopically expressed Inc850 associates with host centrosomes
To further investigate the association between Inc microdomains and host centrosomes, N-terminal mCherry fusions of each microdomain Inc were constructed and used to transfect HeLa cells. In cells expressing mCherry–Inc850, the exogenous protein appeared as aggregates throughout the cell cytoplasm, which showed significant colocalization with the one or both host centrosomes (Fig. 9). This colocalization was observed in cells expressing high, medium or low levels of mCherry–Inc850. Low-level expressing cells are shown for ease of visualization. These results indicate that Inc850, in the absence of a chlamydial inclusion membrane or other chlamydial proteins, is able to associate with host centrosomes. This colocalization persists when cells are infected with C. trachomatis L2 and then transfected with mCherry–Inc850, resulting in centrosomes that colocalized with both the inclusion membrane and exogenous Inc850. In cells expressing mCherry–IncB, –Inc101 or –Inc222, the exogenous protein could be seen in a variety of intracellular structures of different sizes throughout the cytoplasm. However, no significant association with host centrosomes was observed. These results imply that Inc850 may play a key role in the interactions of the C. trachomatis inclusion with the microtubule network.
Quantification of the association of ectopically expressed mCherry–Inc850 with centrosomes indicated that in approximately 98% of the cells expressing mCherry–Inc850, centrosomes were associated with Inc850 (Fig. 10). This is in comparison with approximately 2% of the cells expressing mCherry–IncB.
Within the first few hours following endocytosis, C. trachomatis is unidirectionally transported to a perinuclear location associated with the MTOC where the inclusion remains for the duration of chlamydial intracellular development (Higashi, 1965; Campbell et al., 1989a,b; McBride and Wilde, 1990; Majeed and Kihlstrom, 1991; Hackstadt et al., 1996). Translocation of the nascent inclusion to the MTOC follows microtubule tracks and is mediated by the minus-end directed microtubule motor complex, dynein (Clausen et al., 1997; Grieshaber et al., 2003). Microtubules are organized at the MTOC by centrosomes and the chlamydial inclusion maintains a tight association with centrosomes that is also dependent upon dynein (Grieshaber et al., 2006). Surprisingly, p50 dynamitin, an essential component of the dynactin complex that activates dynein and links vesicular cargo to the dynein motor, is not required for the transport of inclusions to the MTOC or the association of inclusions with centrosomes (Grieshaber et al., 2003; 2006). A requirement for chlamydial de novo transcription and translation to initiate trafficking to the MTOC (Scidmore et al., 1996) has led to the suggestion that chlamydial protein(s) modifying the inclusion membrane may supplant the requirement for p50 dynamitin and an intact dynactin complex in tethering the nascent inclusion to the dynein motor (Grieshaber et al., 2003). The chlamydial proteins mediating this interaction are unknown. Here we describe a novel structure on the chlamydial inclusion membrane that is enriched with active Src family kinases, as well as at least four inclusion membrane proteins, including one that colocalizes with centrosomes when exogenously expressed. Inc microdomains are localized at the point of contact of centrosomes with the inclusion membrane and may represent a complex of chlamydial and host proteins that mediates the interactions with dynein to direct migration along microtubule tracks to the MTOC.
Chlamydia trachomatis expresses up to 50 predicted inclusion membrane proteins characterized by a long, bilobed hydrophobic domain of approximately 40 amino acids in length (Scidmore-Carlson et al., 1999; Bannantine et al., 2000; Shaw et al., 2000; Fields et al., 2003; Li et al., 2008). Incs are exposed on the cytosolic face of the inclusion membrane and thus are likely candidates for factors controlling interactions with the host cell. Incs typically are distributed evenly around the periphery of the inclusion membrane although one has been described as localized to the inclusion membrane at the attachment site of RBs (Hackstadt et al., 1999; Scidmore-Carlson et al., 1999). One additional Inc protein has been described as localized to discrete sites on the inclusion membrane (Alzhanov et al., 2009) although this particular Inc was not one we observed in the microdomains described here. Two of the microdomain Incs, IncB and 850, are early gene products synthesized by 2 h post infection. Inc101 and 222 are considered mid-cycle genes expressed first around 8–12 h post infection (Shaw et al., 2000; Belland et al., 2003). The initial appearance of these microdomains is difficult to visualize because early, nascent inclusions are quite small; however, by 12 h post-infection, when RBs are beginning to divide, microdomains can be clearly observed by immunofluorescent staining (data not shown). These microdomains are observed through at least 36 h post infection. It is worth noting that centripetal migration of nascent inclusions has been initiated by 2 h post infection thus the critical chlamydial proteins mediating interaction with dynein are present and functional by that time (Hackstadt et al., 1996; Grieshaber et al., 2003).
At least two of the Incs, Inc222 and 850, in the microdomain stably interacted with each other in co-immunoprecipitation studies but the other two, IncB and 101, were not similarly associated. It is unclear at this point whether the affinity of Inc222 and 850 is involved in the formation of microdomains or whether other factors may be involved. The chlamydial inclusion is known to contain cholesterol but filipin staining indicates that the microdomains may be further enriched in cholesterol. At this point it cannot be definitively determined whether the microdomain Incs are recruited to a region of high cholesterol or whether the protein composition of these domains instigates the establishment of an altered lipid environment that is enriched in cholesterol.
These microdomains were initially identified based upon the presence of active Src family kinases. A role for tyrosine phosphorylation in chlamydia pathogenesis had been proposed previously based upon the immunofluorescent detection of phosphotyrosine on nascent inclusions although the predicted host protein(s) were not identified (Birkelund et al., 1994; 1997; Clausen et al., 1997; Fawaz et al., 1997). C. trachomatis endocytic vacuoles are now known to be modified by a type III secreted effector protein, Tarp, that is also phosphorylated by Src family kinases (Elwell et al., 2008; Jewett et al., 2008; Lane et al., 2008; Mehlitz et al., 2008). This tyrosine phosphorylation peaks by 30–60 min post infection and diminishes thereafter (Clifton et al., 2004). The function of Tarp tyrosine phosphorylation remains unclear. A clear distinction between Tarp phosphorylation and the intracellular trafficking of nascent inclusions is that the latter requires de novo chlamydial protein synthesis (Scidmore et al., 1996) whereas unphosphorylated Tarp is preloaded in EBs and is translocated and phosphorylated without a requirement for chlamydial transcription or translation (Clifton et al., 2004). It therefore appears likely that tyrosine phosphorylation of either host or chlamydial proteins by Src family kinases may play a role in multiple stages of the chlamydial developmental cycle.
Dynein appears to play an important role in the trafficking of chlamydiae to the MTOC (Clausen et al., 1997; Grieshaber et al., 2003). The absence of a need for the cargo-linking activity of p50 dynamitin to dynein coupled with a requirement for chlamydial modification of the nascent inclusion strongly suggests that a chlamydial protein modifying the inclusion membrane is necessary for intracellular trafficking. Components of the microdomains described here appear to be good candidates based upon their colocalization with centrosomes at the MTOC. Attempts to define specific cellular interactions by immunoprecipitation of each of the four microdomain Incs and immunodetection of components of dynein or dynactin have thus far been unrevealing (data not shown). Although four chlamydial inclusion membrane proteins and host Src family kinases are known to comprise these microdomains, there may be other host or chlamydial proteins present that are required for intracellular interactions. One of the four Incs, Inc850, showed consistent colocalization with centrosomes when ectopically expressed in HeLa cells. Inc850 is expressed as early as 1 h post infection (Belland et al., 2003) and thus is present at the time when trafficking to the MTOC is initiated. These data suggest that Inc850 should bear increased consideration as the possible linkage of the inclusion to the dynein motor. Fyn has been linked with membrane-associated γ-tubulin complexes that can nucleate microtubule formation (Macurek et al., 2008) and has been proposed to play a role in microtubule dynamics and spindle organization (Levi and Shalig, 2010), thus Fyn is also deserving of further attention in consideration of chlamydial interactions with the microtubule network.
The association of the C. trachomatis inclusion with centrosomes is maintained throughout mitosis and can lead to centrosomal abnormalities including an increase in supernumerary chromosomes and chromosomal segregation defects (Grieshaber et al., 2006; Johnson et al., 2009). The chromosomal instability induced by C. trachomatis infection may be maintained even after curing of the infected cells (Grieshaber et al., 2006) and may be a factor in the epidemiological links between chlamydial infection and certain cancers (Hakama et al., 1993; Koskela et al., 2000; Anttila et al., 2001; Wallin et al., 2002; Smith et al., 2004). Identification of the chlamydial factors involved and the mechanisms that mediate the stable interaction of microdomains with centrosomes may shed light on the association of chlamydial infections with certain cancers. C. trachomatis infection also inhibits cytokinesis (Horoschak and Moulder, 1978; Campbell et al., 1989a; Greene and Zhong, 2003). Indeed, three Incs, CT223, 224 and 225, which share little sequence identity among themselves or with any of the microdomain Incs described here, were inhibitory to host cytokinesis when ectopically expressed in McCoy cells (Alzhanov et al., 2009). However, it has also been shown recently that defective cytokinesis cannot entirely account for the increase in centrosome numbers thus other mechanisms must be involved (Johnson et al., 2009).
The unique domain on the C. trachomatis inclusion membrane described here implies a functional interaction with the host microtubule network and the microtubule motor, dynein. These microdomains thus appear to act as a platform for interactions controlling trafficking and positioning of the chlamydial inclusion. A better understanding of this complex should promote a more complete characterization of host and chlamydial proteins involved in this domain and aid in discerning interactions with the host cell promoting infection or subsequent cellular transformation and malignancy.
Organism and cell culture
Chlamydia trachomatis serovar L2 (LGV 434), serovar D (UW-3-Cx), C. muridarum, C. caviae and C. pneumoniae (CWL029) were propagated in HeLa 229 cells and purified by Renografin density gradient centrifugation as previously described (Caldwell et al., 1981).
HeLa cells were plated on glass coverslips in 24-well plates (Corning, Lowell, MA). C. trachomatis L2 and C. caviae infections were performed in Hanks Balanced Salt Solution (HBSS, Invitrogen/Gibco, Carlsbad, CA). After 1 h incubation, the medium was removed and replaced with pre-warmed RPMI-1640/10% FBS. C. trachomatis serovar D and C. muridarum infections were performed as above, except the HeLa cells were pre-treated with 1% DEAE-Dextran in HBSS for 15 min prior to infection. For C. pneumoniae infections, EBs were diluted in SPG buffer (219 mM sucrose, 10 mM Na2HPO4, 3.8 mM KH2PO4, 5 mM glutamic acid, pH 7.4) and centrifuged at 900 g for 1 h. The medium was then replaced with pre-warmed RPMI-1640/10% FBS.
For Fyn, Src and Yes labelling, cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) in PBS, permeabilized with 0.01% saponin (Sigma, St. Louis, MO) in PBS and blocked with 1% BSA (Sigma) in PBS. Cells were then stained with goat anti-Fyn (Novus, Littleton, CO), mouse anti-Src (Millipore/Upstate, Billerica, MA) or mouse anti-Yes (BD Biosciences, San Jose, CA) in conjunction with a polyclonal rabbit anti-EB serum followed by anti-mouse or anti-goat DyLight 594 and anti-rabbit DyLight 488 secondaries (Jackson ImmunoResearch Laboratories, West Grove, PA). For active kinase staining, cells were fixed with cold methanol and labelled with mouse anti-phospho Src family Tyr416 clone 9A6 (Millipore). Note that this antibody was produced against avian Src which is phosphorylated on Tyr416 but reacts with human Src family kinases phosphorylated on Src419. Tubulin staining was performed using mouse anti-β-tubulin (BD Biosciences) and mouse anti-γ-tubulin, clone GTU-88 (Sigma). Dynein intermediate chain was stained with mAb 74.1 (Covance, Emeryville, CA). The chlamydia antibodies used were polyclonal rabbit anti-C. trachomatis L2 EB, anti-C. caviae EB and anti-C. pneumoniae AR39 EB. The anti-IncG antibody has been previously described (Scidmore-Carlson et al., 1999). Monoclonal antibody L2-I-45 against C. trachomatis L2 MOMP was kindly provided by H.D. Caldwell. Rabbit anti-peptide antibodies against IncB (CT232, LARPQVFTLSTQFSPTKPQ, Inc101 (CT101, SKSMLKQHELDAQL), Inc222 (CT222, VRTNYEEVRSSSTGDQV), Inc850 (CT850, TVKDSFLKKARRERFLA) were prepared commercially by Quality Controlled Biochemicals (Hopkinton, MA). The DNA of host cells and chlamydia was counterstained using Draq5 (Biostatus Limited). Cholesterol was visualized using 50 µg ml−1 filipin (Sigma).
Images were acquired on a Nikon Microphot-FXA microscope using a 60× 1.4 NA oil immersion objective (Nikon, USA) or a Zeiss LSM 510 Meta laser confocal scanning microscope using a 63× 1.4 NA oil objective (Carl Zeiss MicroImaging, Maple Grove, MN). Images are representative of typical confocal sections (approximately 0.37 µm).
Transmission electron microscopy
HeLa cells were grown on Thermanox coverslips (Nunc, Rochester, NY) and infected with C. trachomatis L2 for 24 h. Cells were rinsed twice with HBSS and fixed with periodate-lysine-paraformaldehyde (PLP fixative: 75 mM lysine, 37 mM sodium phosphate, 10 mM sodium periodate, 2% paraformaldehyde) plus 0.25% glutaraldehyde for 2 h at room temperature. Samples were rinsed twice with PBS, permeabilized with 0.01% saponin in PBS for 5 min at room temperature, and incubated with mouse anti-active Src kinase (Tyr419) in 0.01% saponin/PBS overnight at room temperature. The cells were rinsed twice with PBS, incubated with peroxidase-conjugated F(ab′)2 donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories) in 0.01% saponin/PBS for 1 h at room temperature and rinsed three times with PBS. The samples were then fixed for 1 h with 1.5% glutaraldehyde in 0.1 M sodium-cacodylate pH 7.4 plus 5% sucrose and rinsed three times in 50 mM Tris-HCl pH 7.4 plus 7.5% sucrose. The reactions were developed using Immunopure Metal Enhanced DAB reagent (Pierce Chemical, Rockford, IL). Samples were rinsed three times with 50 mM Tris-HCl with 7.5% sucrose and fixed overnight at 4°C in 2.5% glutaraldehyde in 100 mM sodium cacodylate buffer, pH 7. Cells were post-fixed twice in 1.0% osmium tetroxide reduced with 1% potassium ferricyanide using a Pelco Biowave laboratory microwave system (Ted Pella, Redding, CA) at 250 W (2 min on, 2 min off, 2 min on) under 20-inch Hg Vacuum. The cells were later washed with water and dehydrated in graded ethanol series for 45 s each in a microwave at 250 W. The cultures were embedded in Spurr's resin and sectioned with a UC6 ultramicrotome (Leica Microsystems). Tomography was performed on the 180 nm thin sections using a Tecnai Spirit TEM (FEI, Hillsboro, OR) at 120 kV. Tilt series with one-degree increments totalling 137 images (−68° to 68°) were recorded on 2048 × 2048 pixel Gatan CCD camera.
siRNA knockdown of Src family kinases
HeLa cells were plated to approximately 50% confluency on coverslips in 24-well plates (Corning). Cells were transfected with ON-TARGETplus SMARTpool siRNA corresponding to Fyn, Src, Yes, Fyn and Src or non-targeting sequence #1 (Thermo Scientific/Dharmacon, Lafayette, CO) according to the manufacturer's instructions. At 48 h after transfection, cells were infected with C. trachomatis serovar L2 and 24 h post infection cells were fixed with methanol for immunostaining. Samples were observed using confocal microscopy and images were acquired using identical settings for each set of samples.
Co-immunoprecipitation and Western blotting
HeLa cells were infected with C. trachomatis serovar L2 or mock-infected. At 24 h post infection, cells were washed three times in cold PBS, scraped into 1.5 ml of 1% Zwittergent 3-14 in PBS, agitated for 20 min on ice, and incubated with protein-A beads followed by centrifugation to pre-clear the lysate. Protein-A agarose beads were incubated with rabbit antibodies against Rickettsia rickettsii, IncB, Inc101, Inc222 or Inc850 for 6 h at room temperature. The beads were rinsed three times with PBS and mixed with the pre-cleared cell lysate. After gentle agitation overnight at 4°C, the beads were washed three times with 1% Zwittergent 3-14 in PBS and proteins were eluted into 1× SDS-PAGE sample buffer. The eluted proteins were separated by SDS-PAGE on a 14% acrylamide gel and electrophoretically transferred to nitrocellulose. Membranes were blocked in 2.5% non-fat dry milk in 50 mM Tris-HCl, pH 7.4 plus 150 mM NaCl (TBS-T) for 1 h and then incubated with anti-IncB, anti-Inc101 or anti-Inc222 in TBS-T overnight at 4°C. Unbound antibody was removed by three rinses with TBS-T and bound antibody detected with an HRP-conjugated donkey anti-rabbit IgG secondary for 45 min. Blots were then rinsed three times with TBS-T and developed with Femto substrate (Pierce) and exposed to CL-Xposure film (Thermo Scientific).
Plasmids and transfections
mCherry was fused to the N-terminus of full-length Inc proteins using the XhoI and EcoRI sites in pmCherry-C1 (Clontech, Mountain View, CA). Each Inc gene was amplified from C. trachomatis serovar L2 DNA using forward primers (IDT, Coralville, IA) that incorporated an XhoI site (IncB: CCC CTC GAG GGA TGG TTC ATT CTG TAT ACA ATT CAT TG; Inc101: CCC CTC GAG GGA TGA TCT CCA TGA TTC CAA GG; Inc222: CCC CTC GAG GGA TGC GTT GCT GTT GTG TTC GTA C; Inc850: CCC CTC GAG GGA TGG GAT TCG GAA CTG TGA GAG G) and reverse primers (IDT) that incorporated an EcoRI site (IncB: CCC GAA TTC CTA TTC TTG AGG TTT TGT TGG GCT G; Inc101: CCC GAA TTC TCA GTA ATA ATA AAC AGA ATA TTT TGA TTT TAA C; Inc222: CCC GAA TTC TCA GTT GGA ATA CAC TAA TTG CTT TTA ATT C; Inc850: CCC GAA TTC TTA CCG ATT CTG GTT GTG AAG TAC TAA C). These plasmids were used to transfect HeLa cells in 24-well plates using Lipofectamine reagents (Invitrogen) according to the manufacturer's instructions.
This work was supported by the intramural research programme of the NIAID/NIH. We thank Janet Sager for technical assistance and Drs R. Heinzen and J. Celli for critical review of the manuscript.