Present address: National Institutes of Health, Bethesda, MD 20892, USA.
Triggering Ras signalling by intracellular Francisella tularensis through recruitment of PKCα and βI to the SOS2/GrB2 complex is essential for bacterial proliferation in the cytosol
Article first published online: 5 JUL 2010
© 2010 Blackwell Publishing Ltd
Volume 12, Issue 11, pages 1604–1621, November 2010
How to Cite
Al-Khodor, S. and Abu Kwaik, Y. (2010), Triggering Ras signalling by intracellular Francisella tularensis through recruitment of PKCα and βI to the SOS2/GrB2 complex is essential for bacterial proliferation in the cytosol. Cellular Microbiology, 12: 1604–1621. doi: 10.1111/j.1462-5822.2010.01494.x
- Issue published online: 5 JUL 2010
- Article first published online: 5 JUL 2010
- Received 16 March, 2010; revised 25 May, 2010; accepted 28 May, 2010.
Fig. S1. Colocalization of Cathepsin-D with the FCP.
A. Representative confocal microscopy images of colocalization of the lysosomal marker Cathepsin-D (red) with the phagosomes containing F. tularensis ssp. novicida (green). Untreated (Un) HEK293T cells or treated with the RNAi-negative control or the gene-specific RNAi for 48 h were infected with the WT strain U112 or formalin-killed (FK) bacteria using moi of 10 for 1 h followed by 1 h treatment with gentamicin to kill extracellular bacteria. The iglC mutant was used as a positive control.
B. Quantitative analysis of colocalization of the WT strain-containing phagosomes with Cathepsin-D at 30 min and 2 h after infection. At least 100 cells were analysed from different coverslips. This experiment was performed three times and the results are representative of one experiment.
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