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Fig. S1. Effect of β-haemolysin on the GBS-III-COH31 induced Rho A, Rac1 and Cdc42 degradation. (A–C) Lysates from control MΦ, MΦ infected with GBS-III-COH31 (MΦ:GBS) at a 1:100 ratio, MΦ infected with hiGBS-III-COH31 (MΦ:hiGBS), MΦ infected with gGBS-III-COH31 (MΦ:gGBS), MΦ incubated with supernatant of GBS-III-COH31 (MΦ:GBS SUP.) growth in culture medium for 2 h at a concentration equivalent to that of MΦ:GBS ratio of 1:100, MΦ infected with GBS-III-COH31 (MΦ:GBS MEM.) at a 1:100 ratio, in contiguous medium separated by a 0.45 μm pore membrane of cell culture insert, MΦ treated with 2 mg ml−1 DPPC, MΦ infected with GBS-III-COH31 (MΦ:GBS) at a 1:100 ratio in the presence of 2 mg ml−1 DPPC, prepared at 2 h, were subjected to SDS-PAGE. Individual filters for each protein were prepared. (A) The filter was probed with anti-Rho A then stripped and reprobed with anti-β-actin. (B) The filter was probed with anti-Rac1 then stripped and reprobed with anti-β-actin. (C) The filter was probed with anti-Cdc42 then stripped and reprobed with anti-β-actin.

Vertical lines in blots in (A)–(C) left panels indicate repositioned gel lanes. The numbers below blots represent the arbitrary units of each protein relative to the densitometric units of β-actin, performed as described in Experimental procedures.

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