Group B Streptococcus (GBS) disrupts by calpain activation the actin and microtubule cytoskeleton of macrophages
Article first published online: 18 MAR 2011
© 2011 Blackwell Publishing Ltd
Volume 13, Issue 6, pages 859–884, June 2011
How to Cite
Fettucciari, K., Quotadamo, F., Noce, R., Palumbo, C., Modesti, A., Rosati, E., Mannucci, R., Bartoli, A. and Marconi, P. (2011), Group B Streptococcus (GBS) disrupts by calpain activation the actin and microtubule cytoskeleton of macrophages. Cellular Microbiology, 13: 859–884. doi: 10.1111/j.1462-5822.2011.01584.x
- Issue published online: 16 MAY 2011
- Article first published online: 18 MAR 2011
- Accepted manuscript online: 8 FEB 2011 04:31AM EST
- Received 7 July, 2010; revised 28 January, 2011; accepted 31 January, 2011.
Fig. S1. Effect of β-haemolysin on the GBS-III-COH31 induced Rho A, Rac1 and Cdc42 degradation. (A–C) Lysates from control MΦ, MΦ infected with GBS-III-COH31 (MΦ:GBS) at a 1:100 ratio, MΦ infected with hiGBS-III-COH31 (MΦ:hiGBS), MΦ infected with gGBS-III-COH31 (MΦ:gGBS), MΦ incubated with supernatant of GBS-III-COH31 (MΦ:GBS SUP.) growth in culture medium for 2 h at a concentration equivalent to that of MΦ:GBS ratio of 1:100, MΦ infected with GBS-III-COH31 (MΦ:GBS MEM.) at a 1:100 ratio, in contiguous medium separated by a 0.45 μm pore membrane of cell culture insert, MΦ treated with 2 mg ml−1 DPPC, MΦ infected with GBS-III-COH31 (MΦ:GBS) at a 1:100 ratio in the presence of 2 mg ml−1 DPPC, prepared at 2 h, were subjected to SDS-PAGE. Individual filters for each protein were prepared. (A) The filter was probed with anti-Rho A then stripped and reprobed with anti-β-actin. (B) The filter was probed with anti-Rac1 then stripped and reprobed with anti-β-actin. (C) The filter was probed with anti-Cdc42 then stripped and reprobed with anti-β-actin.
Vertical lines in blots in (A)–(C) left panels indicate repositioned gel lanes. The numbers below blots represent the arbitrary units of each protein relative to the densitometric units of β-actin, performed as described in Experimental procedures.
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