Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms
Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
Article first published online: 30 JUL 2012
© 2012 Blackwell Publishing Ltd
Volume 14, Issue 11, pages 1734–1744, November 2012
How to Cite
Portevin, D., Via, L. E., Eum, S. and Young, D. (2012), Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype. Cellular Microbiology, 14: 1734–1744. doi: 10.1111/j.1462-5822.2012.01834.x
- Issue published online: 16 OCT 2012
- Article first published online: 30 JUL 2012
- Accepted manuscript online: 12 JUL 2012 07:10AM EST
- Manuscript Accepted: 28 JUN 2012
- Manuscript Revised: 13 JUN 2012
- Manuscript Received: 5 APR 2012
- MRC. Grant Number: U117581288
Fig. S1. NK cells presence in a tuberculous pneumonia sample. At the centre, H&E stain of a section from a tuberculous pneumonia sample resected from the lung of a tuberculous patient that was used for immunofluorescence microscopy assays. Insets from a representative immunostained serial section showing the presence of NK cells (NKp46+ in red) in various part of the lesion, within the consolidated area (top left), within lymphoid aggregates filling alveolar spaces (lower right) and at the border of a well-delimitated necrotic lesion (upper right).
Fig. S2. NK cells presence in a consolidated and necrotizing tuberculous lesion. At the centre, H&E stain of a section from a tuberculous necrotizing granuloma that was used for the following immunofluorescence microscopy assays. Insets from a representative immunostained serial section showing the presence of NK cells (NKp46+ in red) starting notably to infiltrate the epithelioid macrophage layer delimiting a large necrotic lesion.
Fig. S3. NK cells localization in a calcified tuberculous lesion. Top left, H&E stain of a section of a calcified granuloma that was used for the following immunofluorescence microscopy assays. Insets from a representative immunostained serial section reveal the presence of NK cells (NKp46+ in red) recently extravasated from a blood vessel (BV) (inset b), or within surrounding alveolar spaces (inset a, d) of a calcified granuloma. Few signals could be detected at the periphery and infiltrating the sclerotic rim (inset c).
Fig. S4. PBMCs immune response intensity following exposure to M. tuberculosis antigens does not correlate with the ability of respective NK cells to respond to mycobacteria. Histogram comparing IFNγ production from (i) PBMC stimulated with Purified Protein Derivative (PPD) from M. tuberculosis (2 μg ml−1) for 24 h and (ii) NK cell preparation from the matching donor following exposure to M. bovis BCG for 72 h (MOI 1:1) in the presence of IL-2 (100 U ml−1). We observed substantial differences in the intensity of the immune response to PPD among the 52 donors (28 donors below 20 pg ml−1, 16 donors comprised between 20 and 100 pg ml−1 and 8 donors over 100 pg ml−1). However, there was no evident correlation between the memory response to PPD among PBMCs and the variable responsiveness of NK cell exposed to mycobacteria in the presence of co-stimulatory cytokine.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.