Mucosal tumour necrosis factor-alpha in diverticular disease of the colon is overexpressed with disease severity
Copyright © 2011 The Association of Coloproctology of Great Britain and Ireland
- Accepted manuscript online: 21 DEC 2011 07:55AM EST
- Received Date : 12-Jun-2011 , Revised Date : 03-Oct-2011 , Accepted Date : 05-Oct-2011
- Cited By
- diverticular disease;
- segmental colitis associated with diverticulosis;
- tumoral necrosis factor α
Aim: Inflammation occurs in diverticular disease (DD), but there is little information on inflammatory cytokines such as tumour necrosis factor-α (TNF-α). The aim of this study was to assess TNF-α expression in DD and to see whether it is related to the severity of the disease.
Method: Twenty four patients with symptomatic DD were divided into those with acute uncomplicated diverticulitis [AUD], (12 patients) and symptomatic uncomplicated diverticular disease [SUDD] (12 patients). Twelve further patients with asymptomatic diverticulosis (AD), six with segmental colitis associated with diverticulosis (SCAD), with ulcerative colitis (UC) and six healthy individuals (HC) served as controls. TNF-α expression in the colonic mucosa was assessed by the amount of the mRNA codifying for the synthesis of TNF-α.
Results: TNF-α expression was significantly higher in AUD than in HC (p=0.0007), in AD (p=0.0001) and in SUDD (p=0.0179). It was significantly higher also in SUDD than in HC (p=0.0007) and in AD (p=0.0001). TNF-α expression in AUD did not differ significantly from that of UC (p=0.0678) and SCAD (p=0.0610). It was significantly higher in UC, SCAD, and AUD than SUDD (p=0.0007, p=0.0001, p=0.0179).
Conclusion: TNF-α expression in DD seems to be related to the severity of the disease. In particular, it appears to be overexpressed in DD with inflammation (AUD and SUDD) compared with DD without (AD).