Authors contributed equally to this work
Spectral domain optical coherence tomography (SD-OCT) assessment of the healthy female canine retina and optic nerve
Article first published online: 18 APR 2011
© 2011 American College of Veterinary Ophthalmologists
Volume 14, Issue 6, pages 400–405, November 2011
How to Cite
Hernandez-Merino, E., Kecova, H., Jacobson, S. J., Hamouche, K. N., Nzokwe, R. N. and Grozdanic, S. D. (2011), Spectral domain optical coherence tomography (SD-OCT) assessment of the healthy female canine retina and optic nerve. Veterinary Ophthalmology, 14: 400–405. doi: 10.1111/j.1463-5224.2011.00887.x
- Issue published online: 14 NOV 2011
- Article first published online: 18 APR 2011
- canine retina;
- nerve fiber layer;
- optical coherence tomography;
- outer nuclear layer;
- photoreceptor layer;
- retinal thickness
Objective To provide normative data for canine whole retinal thickness (WRT), nerve fiber layer thickness (NFL), photoreceptor layer thickness (PR), and outer nuclear layer thickness (ONL) using spectral domain optical coherence tomography. Animal studied: Twelve healthy adult intact female beagles.
Procedure Horizontal volume scans through the area dorso-temporal from the optic nerve (superior retina), and the area ventro-temporal from the optic nerve (inferior retina) were used to evaluate the thickness of retinal NFL, PR, ONL, and WRT. Peripapillary circular scans were used to evaluate NFL thickness. Statistical analyses were performed to compare the thickness of the individual layers between the superior and inferior retina (paired t-test). One-way analysis of variance (ANOVA) was used to compare the thickness of peripapillary NFL between the superior, inferior, temporal and nasal quadrants of the circle scan.
Results The WRT, PR, and NFL thickness were greater in the superior than in the inferior retina (198.7 ± 9.6 μm vs. 164.4 ± 6.4 μm, P < 0.0001; 95.5 ± 6.5 μm vs. 78.8 ± 7.4 μm, P < 0.0001; and 26.4 ± 1.6 μm vs. 25.0 ± 1.9 μm, P = 0.0236, respectively). No statistical difference was found between the ONL thickness of the superior and inferior retina (50.1 ± 6.4 μm vs. 44.3 ± 3.6, P = 0.0578). Peripapillary NFL thickness showed a similar tendency as the linear scans, with the superior quadrant having the greatest thickness (91.26 ± 7.0 μm) and the inferior quadrant being the thinnest (76.42 ± 9.2 μm) (P < 0.001).
Conclusions Results of our in vivo studies showed significant differences between thickness values for the superior (tapetal) and inferior (nontapetal) retinal regions.