The work has been presented as a poster in (European Association for Vision and Eye Research) EVER, Greece in October 2010.
Ultrastructure features of camel cornea – collagen fibril and proteoglycans
Article first published online: 12 JUL 2011
© 2011 American College of Veterinary Ophthalmologists
Volume 15, Issue 1, pages 36–41, January 2012
How to Cite
Almubrad, T. and Akhtar, S. (2012), Ultrastructure features of camel cornea – collagen fibril and proteoglycans. Veterinary Ophthalmology, 15: 36–41. doi: 10.1111/j.1463-5224.2011.00918.x
- Issue published online: 18 JAN 2012
- Article first published online: 12 JUL 2011
- collagen fibrils;
Introduction The uniform distribution of collagen fibrils and proteoglycans maintain the transparency of normal cornea. We describe the ultrastructural features of camel cornea including collagen fibrils and proteoglycans (PGs).
Methods Camel corneas (of 6-, 8-, and 10-month-old animals) were fixed in 2.5% glutaraldehyde containing cuprolinic blue in sodium acetate buffer and processed for electron microscopy. The ‘AnalySIS LS Professional’ program was used to analyze the collagen fibril diameter.
Results The camel cornea consists of four layers: the epithelium (227 μm), stroma (388 μm), Descemet’s membrane (DM), and endothelium. The epithelium constituted 36% of the camel cornea, whereas corneal stroma constituted 62% of the corneal thickness (629 μm). The PGs in the posterior stroma were significantly larger in number and size compared with the anterior and middle stroma. The collagen fibril diameter was 25 nm and interfibrillar spacing 40 nm. Fibrillar structures are present throughout the DM.
Conclusion The structure of the camel cornea is very different from human and other animals. The unique structure of the cornea might be an adaptation to help the camel to survive in a hot and dry climate. The camel cornea may also be a good model to study the effect of hot and dry climates on the cornea.