Normal corneal thickness measurements in pigmented rabbits using spectral-domain anterior segment optical coherence tomography
Article first published online: 6 JUN 2012
© 2012 American College of Veterinary Ophthalmologists
Volume 16, Issue 2, pages 130–134, March 2013
How to Cite
Wang, X. and Wu, Q. (2013), Normal corneal thickness measurements in pigmented rabbits using spectral-domain anterior segment optical coherence tomography. Veterinary Ophthalmology, 16: 130–134. doi: 10.1111/j.1463-5224.2012.01041.x
- Issue published online: 5 MAR 2013
- Article first published online: 6 JUN 2012
- anterior segment;
- optical coherence tomography;
- pigmented rabbit;
Objective To document the thickness of the central cornea in pigmented rabbits using spectral-domain anterior segment optical coherence tomography (AS-OCT).
Animals studied Seventeen pigmented rabbits (6 male, 11 female, both eyes) were involved in this study.
Procedures Thirty-four eyes from healthy pigmented rabbits underwent a complete ophthalmologic examination, including AS-OCT. Eight radial scans, 6 mm in length and centered on the cornea, were obtained using the AS-OCT. Corneal thickness was automatically calculated using pachymetry software. Measurements were displayed as the mean and standard deviation for each of the 17 regions defined by the software. The regions were the center (1 mm radius, area a), the inner ring (2.5 mm radius, area b), the outer ring (3 mm radius, area c), and the eight radial scan lines in eight directions (Superior (1), SN (2), Nasal (3), IN (4), Inferior (5), IT (6), Temporal (7), ST (8)) with an angle of 45° between each consecutive scan line (a, b 1–8, c 1–8).
Results There was no statistically significance difference in corneal thickness between gender, eye, and the eight directions (P = 0.804, P > 0.05, P > 0.05). There was a statistical difference between the thickness in areas a, b, and c (P < 0.05). The corneal thickness increased gradually from the center to the periphery of the 6 mm measured. The center corneal thickness was 387 ± 19.8 μm for OD and 384 ± 20.2 μm for OS. The corneal thicknesses of the thinnest point of the right eyes (OD) and the left eyes (OS) were 376 ± 20.2 and 370 ± 16.8 μm, respectively. There was positive correlation between the thinnest point and area a in both the right eyes (r = 0.892, P < 0.001) and the left eyes (r = 0.832, P < 0.001).
Conclusions This is the first documentation of the rabbit corneal thickness in vivo using the spectral-domain AS-OCT. Pigmented rabbit corneas were almost 150 μm thinner than human corneal values. Gender and eye were not associated with any statistical differences in central corneal thickness in this study.