Anterior segment fluorescein angiography of the normal feline eye using a dSLR camera adaptor

Authors


  • Presented as a poster at the 42nd Annual Meeting of the American College of Veterinary Ophthalmologists, Hilton Head, SC, USA, October 2011.

  • Funding source(s): None.

  • AF Alario (None), CG Pirie (P), S Pizzirani (None).

Address communications to:
C. Pirie
Tel.: +1508 839 5395
Fax: +1 508 887 4363
e-mail: chris.pirie@tufts.edu

Abstract

Purpose  To describe anterior segment fluorescein angiography (ASFA) of the normal feline eye using a digital single-lens reflex (dSLR) camera adaptor.

Animals  Ten cats free of ocular and systemic disease were evaluated.

Methods  All cats received maropitant citrate (1.0 mg/kg SQ) and diphenhydramine (2.0 mg/kg SQ) 20 min prior to anesthesia using propofol (4 mg/kg IV bolus, 0.2 mg/kg/min CRI). Standard color and red-free images were obtained prior to the administration of 10% sodium fluorescein (20 mg/kg IV). Imaging was performed using a dSLR camera (Canon 7D), dSLR camera adaptor, camera lens (Canon EF-S 60 mm f/2.8 macro), and an accessory flash (Canon 580EXII). Imaging occurred at a rate of 1/second immediately following IV bolus of sodium fluorescein for a total of 30 s, then at 1, 2, 3, 4, 5, and 10 min.

Results  Ten cats with an average age of 3.7 ± 0.9 years and various iris colors were imaged. Arterial, capillary, and venous phases occurred 4.6, 7.8, and 8.9 s postinjection, respectively. Visibility of the vasculature was not impaired by the degree of iris pigmentation. Patency of a persistent pupillary membrane was noted in one cat. Vessel leakage was common, as well as, leakage into the aqueous humor. Proper patient positioning and restricted ocular movements were critical. No adverse events were noted.

Conclusions  This study demonstrates ASFA findings in normal feline eyes using a cost-effective dSLR camera adaptor. Fluorescein leakage from vessels and into the aqueous humor was a common finding. Visibility of iris vasculature was not impaired by the degree of iris pigmentation.

Ancillary