Oestrogen receptor expression and neuronal nitric oxide synthase in the clitoris and preputial gland structures of mice
Article first published online: 12 SEP 2008
© 2008 THE AUTHORS. JOURNAL COMPILATION © 2008 BJU INTERNATIONAL
Volume 102, Issue 11, pages 1719–1723, December 2008
How to Cite
Martin-Alguacil, N., Schober, J., Kow, L.-M. and Pfaff, D. (2008), Oestrogen receptor expression and neuronal nitric oxide synthase in the clitoris and preputial gland structures of mice. BJU International, 102: 1719–1723. doi: 10.1111/j.1464-410X.2008.07989.x
- Issue published online: 21 NOV 2008
- Article first published online: 12 SEP 2008
- Accepted for publication 10 April 2008
- oestrogen receptor α;
- neuronal nitric oxide synthase;
To study the presence of oestrogen receptors (ER) and neuronal nitric oxide synthase (nNOS) in the mouse clitoris.
MATERIALS AND METHODS
A series of sections of the pelvic area, including the preputial glands and clitoris, of 10 mice were assessed by immunocytochemical studies specific for ER-α and -β, and nNOS; selected sections were also stained with Masson’s trichrome.
ERα was detected in the epithelium of the gland of the clitoris, and in the glandular tissue, preputial and apocrine gland. ERα was detected in the nuclei of stromal cells around the cavernous tissue and near the epithelium of the clitoris. Cytoplasm ERα was detected in a few cells in an area ventral to the clitoral gland. There was also nuclear staining in the connective tissue cells surrounding the clitoris. Very light ERβ immunostaining was detected in the clitoris and in the tissue related to it. There were some cells with nuclear staining in the vessels of the cavernous tissue of the clitoris. nNOS immunostaining was detected in the clitoris, the preputial gland and the connective tissue.
ERα and β isoforms, and nNOS, are present in the clitoris and preputial glands of female mice in different cellular locations and with differing levels of receptivity. Functional studies would further elucidate the role of receptor functions and their relationship to the neuronal expression of NO.