Vitamin E (α-tocopherol) supplementation enhances nitric oxide production in penile tissue of diabetic rats
Article first published online: 15 NOV 2010
© 2010 BJU INTERNATIONAL. NO CLAIM TO ORIGINAL US GOVERNMENT WORKS
Volume 106, Issue 11, pages 1788–1793, December 2010
How to Cite
Tronchini, E. A., De Miranda Neto, M. H. and Zanoni, J. N. (2010), Vitamin E (α-tocopherol) supplementation enhances nitric oxide production in penile tissue of diabetic rats. BJU International, 106: 1788–1793. doi: 10.1111/j.1464-410X.2010.09312.x
- Issue published online: 15 NOV 2010
- Article first published online: 15 NOV 2010
- Accepted for publication 22 December 2009
- diabetes mellitus;
- penile tissue;
- vitamin E
To investigate the effects of 0.1% and 2% vitamin E (α-tocopherol) supplementation on the expression of nitric oxide (NO) in penile tissue of rats with experimentally induced diabetes mellitus (DM).
MATERIALS AND METHODS
In all, 30 male rats were divided into six groups: normoglycaemic (NG), NG treated with 0.1% vitamin E (NGE1), NG treated with 2% vitamin E (NGE2), DM, DM treated with 0.1% vitamin E (DME1), and DM treated with 2% vitamin E (DME2). After 120 days the rats were killed, and penile tissue was collected and processed for neuronal NO synthase (nNOS) immunohistochemistry to determine areas of nNOS-immunoreactive varicosities.
nNOS-immunoreactive varicosities in DME2 rats were similar to those of controls (NG) and controls supplemented with vitamin E (NGE1 and NGE2). Varicosity sizes in the NGE1 group were similar to the DM rats with no vitamin E supplementation.
Supplementation with 2% vitamin E had a positive effect on areas of nNOS-immunoreactive varicosities of penile tissue in DM rats.