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FIG. S1. Experimental workflow for mRNA expression analysis of FFPE 189 lymph node regions specimens.

Histological sections of archived paraffin blocks of the same LNR are cut and placed in a xylene-filled microtube for deparaffinization. After ethanol wash and protease digestion, RNA is isolated. RNA quantity and quality are measured and RNA is reverse transcribed to cDNA. Then, cDNA is pre-amplified and finally quantified by real-time quantitative PCR.

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