L.M. and A.A. contributed equally to the work.
Biomarkers vs conventional histological analysis to detect lymph node micrometastases in bladder cancer: a real improvement?
Article first published online: 15 MAR 2012
© 2012 BJU INTERNATIONAL
Volume 110, Issue 9, pages 1310–1316, November 2012
How to Cite
Gazquez, C., Ribal, M. J., Marín-Aguilera, M., Kayed, H., Fernández, P. L., Mengual, L. and Alcaraz, A. (2012), Biomarkers vs conventional histological analysis to detect lymph node micrometastases in bladder cancer: a real improvement?. BJU International, 110: 1310–1316. doi: 10.1111/j.1464-410X.2012.11025.x
- Issue published online: 16 OCT 2012
- Article first published online: 15 MAR 2012
- Accepted for publication 20 December 2011
FIG. S1. Experimental workflow for mRNA expression analysis of FFPE 189 lymph node regions specimens.
Histological sections of archived paraffin blocks of the same LNR are cut and placed in a xylene-filled microtube for deparaffinization. After ethanol wash and protease digestion, RNA is isolated. RNA quantity and quality are measured and RNA is reverse transcribed to cDNA. Then, cDNA is pre-amplified and finally quantified by real-time quantitative PCR.
|bju11025_sm_FigS1.ppt||118K||Supporting info item|
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