• 1
    Devroey P, Liu J, Nagy Z, Tournaye H, Silber SJ, Van Steirteghem AC. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil Steril 1994; 62: 639641
  • 2
    Coughlan C, Ledger W. In-vitro fertilisation. Obstet Gynaecol Reprod Med 2008; 18: 300306
  • 3
    Gougeon A. The biological aspects of risks of infertility due to age: the female side. Rev Epidemiol Sante Publique 2005; 53 (Spec No 2): 2S37245
  • 4
    Desai N, AbdelHafez F, Sabanegh E, Goldfarb J. Paternal effect on genomic activation, clinical pregnancy and live birth rate after ICSI with cryopreserved epididymal versus testicular spermatozoa. Reprod Biol Endocrinol 2009; 7: 142147
  • 5
    Nicopoullos JD, Gilling-Smith C, Almeida PA, Norman-Taylor J, Grace I, Ramsay JW. Use of surgical sperm retrieval in azoospermic men: a meta-analysis. Fertil Steril 2004; 82: 691701
  • 6
    Abdel Raheem A, Garaffa G, Rushwan N et al. Testicular histopathology as a predictor of a positive sperm retrieval in azoospermic men. BJU Int 2012. [Epub ahead of print]. doi: 10.1111/j.1464-410X.2012.11203.x
  • 7
    Giorgetti C, Chinchole JM, Hans E et al. Crude cumulative delivery rate following ICSI using intentionally frozen-thawed testicular spermatozoa in 51 men with non-obstructive azoospermia. Reprod Biomed Online 2005; 11: 319324
  • 8
    Matyas S, Papp G, Kovacs P, Balogh I, Rajczy K, Kopa Z. the Hungarian experience). Intracytoplasmic injection with motile and immotile frozen-thawed testicular spermatozoa. Andrologia 2005; 37: 2528
  • 9
    Huang FJ, Chang SY, Tsai MY et al. Clinical implication of intracytoplasmic injection using cryopreserved testicular spermatozoa from men with azoospermia. J Reprod Med 2000; 45: 310316
  • 10
    Ishikawa T, Shiotani M, Izumi Y et al. Fertilization and pregnancy using cryopreserved testicular sperm for intracytoplasmic sperm injection with azoospermia. Fertil Steril 2009; 92: 174179
  • 11
    Holden CA, Fuscaldo GF, Jackson P et al. Frozen-thawed epididymal spermatozoa for intracytoplasmic sperm injection. Fertil Steril 1997; 67: 8187
  • 12
    Thompson-Cree ME, McClure N, Donnelly ET, Steele KE, Lewis SE. Effects of cryopreservation on testicular sperm nuclear DNA fragmentation and its relationship with assisted conception outcome following ICSI with testicular spermatozoa. Reprod Biomed Online 2003; 7: 449455
  • 13
    Ulug U, Bener F, Karagenc L, Ciray N, Bahceci M. Outcomes in couples undergoing ICSI: comparison between fresh and frozen-thawed surgically retrieved spermatozoa. Int J Androl 2005; 28: 343349
  • 14
    Gilmore JA, Liu J, Peter AT, Critser JK. Determination of plasma membrane characteristics of boar spermatozoa and their relevance to cryopreservation. Biol Reprod 1998; 58: 2836
  • 15
    Nogueira D, Bourgain C, Verheyen G, Van Steirteghem AC. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies. Hum Reprod 1999; 14: 20412049
  • 16
    Verheyen G, Nagy Z, Joris H, De C, Tournaye IH, Van SA. Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal vesicle stage oocytes. Fertil Steril 1997; 67: 7480
  • 17
    Chatterjee S, Gagnon C. Production ofreactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 2001; 59: 451458
  • 18
    Chan PT, Schlegel PN. Non-obstructive azoospermia. Curr Opin Urol 2000; 10: 617624
  • 19
    Vicari E, Grazioso C, Burrello N, Cannizzaro M, D'Agata R, Calogero AE. Epididymal and testicular sperm retrieval in azoospermic patients and the outcome of intracytoplasmic sperm injection in relation to the etiology of azoospermia. Fertil Steril 2001; 75: 215216