GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination
Article first published online: 15 MAR 2007
Volume 24, Issue 5, pages 521–526, May 2007
How to Cite
Bekris, L. M., Jensen, R. A., Lagerquist, E., Hall, T. R., Agardh, C.-D., Cilio, C. M., Lethagen, Å. L., Lernmark, Å., Robertson, J. A. and Hampe, C. S. (2007), GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination. Diabetic Medicine, 24: 521–526. doi: 10.1111/j.1464-5491.2007.02091.x
- Issue published online: 22 MAR 2007
- Article first published online: 15 MAR 2007
- Accepted 29 October 2007
- Type 1 diabetes
Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response.
Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 µg alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins.
Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-µg dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65.
Conclusions Our data suggest that the apparent beneficial effects of 20 µg alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern.