The aim of the study was to evaluate the efficacy of polysaccharides from fish cartilage with regard to their skin aging properties. An application test was carried out during the intake of cartilage tablets as a nutritional supplement. The study was designed as a monocentric, double-blind, randomized, placebo-controlled application test. 28 healthy female volunteers (35–60 years) with dry skin were included in the study. They were divided into two groups. Group 1 received tablets containing placebo and group 2 the verum treatment (cartilage hydrolysate combined with vitamin C). The duration of the study was 12 weeks. The focus of interest was to find out about the hydration properties, and to see whether the skin barrier and structure were influenced by the test formulation compared with placebo. Hydration measurements were made before and during the study, and the transepidermal water loss (barrierer function of the skin) was measured. The thickness and density of the skin of all volunteers were determined by means of ultrasound measurements during the study. Statistical analysis was based on Wilcoxon signed rank test. The following results were obtained in this study: there was a significant improvement in the hydration properties, a significant decrease in transepidermal water loss and a significant increase in the skin density in the verum group (cartilage extract). No or minor improvements could be detected in the placebo group.