ABSTRACT This study examined sexual prejudice and masculine gender role stress as mediators of the relations between male gender norms and anger and aggression toward gay men. Participants were 150 self-identified heterosexual men who completed measures of adherence to male gender role norms, sexual prejudice, masculine gender role stress, and state anger. Participants then viewed a video depicting intimate relationship behavior between 2 gay men, reported state anger a second time, and competed in a laboratory aggression task against either a heterosexual or a gay male. Results indicated that adherence to the antifemininity norm exerted an indirect effect, primarily through sexual prejudice, on increases in anger. Adherence to the status and antifemininity norms exerted indirect effects, also through sexual prejudice, on physical aggression toward the gay, but not the heterosexual, male. Findings provide the first multivariate evidence for determinants of aggression toward gay men motivated by gender role enforcement.