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  • Agros, P. (1990) An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion. J. Mol. Biol. 211, 943958.
  • Bartolome, B., Jubete, Y., Martinez, E. and De La Cruz, F. (1991) Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene, 102, 7578.
  • Beaujean, A., Ducrocq-Assaf, C., Sangwan, R.S., Lilius, G., Bulow, L. and Sangwan-Norreel, B.S. (2000) Engineering direct fructose production in processed potato tubers by expressing a bifunctional α-amylase/glucose isomerase gene complex. Biotechnol. Bioeng. 70, 916.
  • Bevan, M., Barnes, W.M. and Chilton, M.-D. (1983) Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 11, 369385.
  • Bohmert, K., Balbo, I., Kopka, J., Mittendorf, V., Nawrath, C., Poirier, Y., Tischendorf, G., Trethewey, R.N. and Willmitzer, L. (2000) Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight. Planta, 211, 841845.
  • Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72, 248254.
  • Bulow, L. and Mosbach, K. (1991) Multienzyme systems obtained by gene fusion. Trends Biotechnol. 9, 226231.
  • Carlsson, H., Ljung, S. and Bulow, L. (1996) Physical and kinetic effects on induction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymes. Biochim. Biophys. Acta, 1293, 154160.
  • Cashmore, A.R. (1983) Nuclear genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. In: Genetic Engineering of Plants: An Agricultural Perspective (Kosuge, T., Meredith, C.P. and Hollaender, A., eds), pp. 2938. New York: Plenum Publications Corp.
  • Chiu, W., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H. and Sheen, J. (1996) Engineered GFP as a vital reporter in plants. Curr. Biol. 6, 325330.
  • Clough, S.J. and Bent, A.F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735743.
  • Davis, J.T., Moore, R.N., Imperiali, B., Pratt, A.J., Kobayashi, K., Masamune, S., Sinskey, A.J., Walsh, C.T., Fukui, T. and Tomita, K. (1987) Biosynthetic thiolase from Zoogloea ramigera. I. Preliminary characterization and analysis of proton transfer reaction. J. Biol. Chem. 262, 8289.
  • De Wilde, C., Van Houdt, H., De Buck, S., Angenon, G., De Jaeger, G. and Depicker, A. (2000) Plants as bioreactors for protein production: avoiding the problem of transgene silencing. Plant Mol. Biol. 43, 347359.
  • El Amrani, A., Barakate, A., Askari, B.M., Li, X., Roberts, A.G., Ryan, M.D. and Halpin, C. (2004) Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiol. 135, 1624.
  • Francois, I.E.J.A., Broekaert, W.F. and Cammue, B.P.A. (2002a) Different approaches for multi-transgene-stacking in plants. Plant Sci. 163, 281295.
  • Francois, I.E.J.A., De Bolle, M.F.C., Dwyer, G., Goderis, I.J.W.M., Woutors, P.F.J., Verhaert, P.D., Proost, P., Schaaper, W.M.M., Cammue, B.P.A. and Broekaert, W.F. (2002b) Transgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. Plant Physiol. 128, 13461358.
  • Garg, A.K., Kim, J.-K., Owens, T.G., Ranwala, A.P., Choi, Y.D., Kochian, L.V. and Wu, R.J. (2002) Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc. Natl. Acad. Sci. USA, 99, 15 89815 903.
  • Gerngross, T.U., Reilly, P., Stubbe, J., Sinskey, A.J. and Peoples, O.P. (1993) Immunocytochemical analysis of poly-β-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules. J. Bacteriol. 175, 52895293.
  • Gerngross, T.U., Snell, K.D., Peoples, O.P., Sinskey, A.J., Csuhai, E., Masamune, S. and Stubbe, J. (1994) Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry, 33, 93119320.
  • Halpin, C., Barakate, A., Askari, B.M., Abbott, J.C. and Ryan, M.D. (2001) Enabling technologies for manipulating multiple genes on complex pathways. Plant Mol. Biol. 47, 295310.
  • Houmiel, K.L., Slater, S., Broyles, D., Casagrande, L., Colburn, S., Gonzalez, K., Mitsky, T.A., Reiser, S.E., Shah, D., Taylor, N.B., Tran, M., Valentin, H.E. and Gruys, K.J. (1999) Poly(β-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus. Planta, 209, 547550.
  • Huisman, G.W., Skraly, F., Martin, D.P. and Peoples, O.P. (2001) Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids. US Patent 6,316,262.
  • Hunt, A.G. and Maiti, I.B. (2001) Strategies for expressing multiple foreign genes in plants as polycistronic constructs. In Vitro Cell. Dev. Biol. Plant, 37, 313320.
  • Jang, I.-C., Oh, S.-J., Seo, J.-S., Choi, W.-B., Song, S.I., Kim, C.H., Kim, Y.S., Seo, H.-S., Choi, Y.D., Nahm, B.H. and Kim, J.-K. (2003) Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol. 131, 516524.
  • Kim, G., Cheon, Y. and Kim, H. (2000a) Directed evolution of a novel N-carbamylase/d-hydantoinase fusion enzyme for functional expression with enhanced stability. Biotechnol. Bioeng. 68, 211217.
  • Kim, G.-J., Lee, D.-E. and Kim, H.-S. (2000b) Construction and evaluation of a novel bifunctional N-carbamylase-d-hydantoinase fusion enzyme. Appl. Environ. Microbiol. 66, 21332138.
  • Kim, Y.B. and Lenz, R.W. (2001) Polyesters from microorganisms. Adv. Biochem. Eng. Biotechnol. 71, 5279.
  • Koncz, C. and Schell, J. (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet. 204, 383396.
  • Lindbladh, C., Rault, M., Hagglund, C., Small, W., Mosbach, K., Bulow, L., Evans, C. and Srere, P. (1994) Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase. Biochemistry, 33, 11 69211 698.
  • Ljungcrantz, P., Bulow, L. and Mosbach, K. (1990) Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/β-galactosidase/galactokinase. FEBS Lett. 275, 9194.
  • Ljungcrantz, P., Carlsson, H., Mansson, M., Buckel, P., Mosbach, K. and Bulow, L. (1989) Construction of an artificial bifunctional enzyme, β-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling. Biochemistry, 28, 87868792.
  • Lössl, A., Eibl, C., Harloff, H.J., Jung, C. and Koop, H.-U. (2003) Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction. Plant Cell Report, 21, 891899.
  • Madison, L.L. and Huisman, G.W. (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63, 2153.
  • Mao, Q., Schunk, T., Gerber, B. and Erni, B. (1995) A string of enzymes, purification and characterization of a fusion protein comprising the four subunits of the glucose phosphotransferase system of Escherichia coli. J. Biol. Chem. 270, 18 29518 300.
  • Marchesini, S., Erard, N., Glumoff, T., Hiltunen, J.K. and Poirier, Y. (2003) Modification of the monomer composition of polyhydroxyalkanoate synthesized in Saccharomyces cerevisiae expressing variants of the β-oxidation-associated multifunctional enzyme. Appl. Environ. Microbiol. 69, 64956499.
  • Masamune, S., Walsh, C.T., Sinskey, A.J. and Peoples, O.P. (1989) Poly-(R)-3-hydroxybutyrate (PHB) biosynthesis: mechanistic studies on the biological Claisen condensation catalyzed by β-ketoacyl thiolase. Pure Appl. Chem. 61, 303312.
  • Nawrath, C., Poirier, Y. and Somerville, C. (1994) Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Proc. Natl. Acad. Sci. USA, 91, 12 76012 764.
  • Nixon, A., Ostermeier, M. and Benkovic, S. (1998) Hybrid enzymes: manipulating enzyme design. Trends Biotechnol. 16, 258264.
  • Palmer, M.A.J., Differding, E., Gamboni, R., Williams, S.F., Peoples, O.P., Walsh, C.T., Sinskey, A.J. and Masamune, S. (1991) Biosynthetic thiolase from Zoogloea ramigera: evidence for a mechanism involving Cys-378 as the active site base. J. Biol. Chem. 266, 17.
  • Peoples, O.P., Madison, L.L. and Huisman, G.W. (2000) Enzymes for biopolymer production. International Patent Application WO 00/06747.
  • Peoples, O.P. and Sinskey, A. (1989a) Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding β-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Mol. Microbiol. 3, 349357.
  • Peoples, O.P. and Sinskey, A.J. (1989b) Poly-3-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16: characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reductase. J. Biol. Chem. 264, 15 29315 297.
  • Peoples, O.P. and Sinskey, A.J. (1989c) Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16: identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264, 15 29815 303.
  • Pettersson, H., Olsson, P., Bulow, L. and Pettersson, G. (2000) Kinetics of the coupled reaction catalysed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase. Eur. J. Biochem. 267, 50415046.
  • Ploux, O., Masamune, S. and Walsh, C.T. (1988) The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera: characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli. Eur. J. Biochem. 174, 177182.
  • Poirier, Y., Dennis, D.E., Klomparens, K. and Somerville, C. (1992) Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science, 256, 520523.
  • Poirier, Y. and Gruys, K.J. (2002) Production of polyhydroxyalkanoates in transgenic plants. In: Biopolymers (SteinbüchelA., ed.), pp. 401435. Weinheim: Wiley-VHC-Verlag GmbH.
  • Riedel, K. and Bronnenmeier, K. (1998) Intramolecular synergism in an engineered exo-endo-1,4-β-glucanase fusion protein. Mol. Microbiol. 28, 767775.
  • Seo, H.S., Koo, Y.J., Lim, J.Y., Song, J.T., Kim, C.H., Kim, J.K., Lee, J.S. and Choi, Y.D. (2000) Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli. Appl. Environ. Microbiol. 66, 24842490.
  • Snell, K.D., Feng, F., Zhong, L., Martin, D. and Madison, L.L. (2002) YfcX enables medium chain length poly(3-hydroxyalkanoate) formation from fatty acids in recombinant Escherichia coli fadB strains. J. Bacteriol. 184, 56965705.
  • Snell, K.D. and Peoples, O.P. (2002) Polyhydroxyalkanoate polymers and their production in transgenic plants. Metab. Eng. 4, 2940.
  • Steinbuchel, A. and Hein, S. (2001) Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in microorganisms. Adv. Biochem. Eng. Biotechnol. 71, 82123.
  • Steinbuchel, A. and Valentin, H.E. (1995) Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol. Lett. 128, 219228.
  • Sudesh, K., Abe, H. and Doi, Y. (2000) Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 25, 15031555.
  • Sumner, L.W., Mendes, P. and Dixon, R.A. (2003) Plant metabolomics: large-scale phytochemistry in the functional genomics era. Phytochemistry, 62, 817836.
  • Taguchi, K., Taguchi, S., Sudesh, K., Maehara, A., Tsuge, T. and Doi, Y. (2002) Metabolic pathways and engineering of PHA biosynthesis. Biopolymers, 3a, 217247.
  • Terentiev, Y., Breuer, U., Babel, W. and Kunze, G. (2004) Non-conventional yeasts as producers of polyhydroxyalkanoates – genetic engineering of Arxula adeninivorans. Appl. Microbiol. Biotechnol. 64, 376381.
  • Trethewey, R.N. (2004) Metabolite profiling as an aid to metabolic engineering in plants. Curr. Opin. Plant Biol. 7, 196201.
  • Turner, N.J. (2003) Directed evolution of enzymes for applied biocatalysis. Trends Biotechnol. 21, 474478.
  • Urwin, P.E., Atkinson, H.J. and Zubko, E.I. (2002) The biotechnological application and limitation of IRES to deliver multiple defense genes to plant pathogens. Physiol. Mol. Plant Pathol. 61, 103108.
  • Urwin, P.E., McPherson, M.J. and Atkinson, H.J. (1998) Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs. Planta, 204, 472479.
  • Williams, M.D., Rahn, J.A. and Sherman, D.H. (1996) Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eukaryotic fatty acid synthase. Appl. Environ. Microbiol. 62, 25402546.
  • Wrobel, M., Zebrowski, J. and Szopa, J. (2004) Polyhydroxybutyrate synthesis in transgenic flax. J. Biotechnol. 107, 4154.
  • Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103119.
  • Yilmaz, J.L. and Bulow, L. (2002) Enhanced stress tolerance in Escherichia coli and Nicotiana tabacum expressing a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein. Biotechnol. Prog. 18, 11761182.