Present address: Plant Biotechnology Division, IFRB, AERE Bangladesh Atomic Energy Commission, GPO Box No. 3787, Dhaka-1000, Bangladesh
Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen
Article first published online: 27 APR 2007
Plant Biotechnology Journal
Volume 5, Issue 4, pages 483–494, July 2007
How to Cite
Ribarits, A., Mamun, A. N. K., Li, S., Resch, T., Fiers, M., Heberle-Bors, E., Liu, C.-M. and Touraev, A. (2007), Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen. Plant Biotechnology Journal, 5: 483–494. doi: 10.1111/j.1467-7652.2007.00256.x
- Issue published online: 27 APR 2007
- Article first published online: 27 APR 2007
- Received 12 December 2006; revised 27 February 2007; accepted 1 March 2007.
- doubled haploid plants;
- glutamine synthetase;
- male sterility;
Reversible male sterility and doubled haploid plant production are two valuable technologies in F1-hybrid breeding. F1-hybrids combine uniformity with high yield and improved agronomic traits, and provide self-acting intellectual property protection. We have developed an F1-hybrid seed technology based on the metabolic engineering of glutamine in developing tobacco anthers and pollen. Cytosolic glutamine synthetase (GS1) was inactivated in tobacco by introducing mutated tobacco GS genes fused to the tapetum-specific TA29 and microspore-specific NTM19 promoters. Pollen in primary transformants aborted close to the first pollen mitosis, resulting in male sterility. A non-segregating population of homozygous doubled haploid male-sterile plants was generated through microspore embryogenesis. Fertility restoration was achieved by spraying plants with glutamine, or by pollination with pollen matured in vitro in glutamine-containing medium. The combination of reversible male sterility with doubled haploid production results in an innovative environmentally friendly breeding technology. Tapetum-mediated sporophytic male sterility is of use in foliage crops, whereas microspore-specific gametophytic male sterility can be applied to any field crop. Both types of sterility preclude the release of transgenic pollen into the environment.