• Open Access

Coordinated regulation of maize genes during increasing exposure to ultraviolet radiation: identification of ultraviolet-responsive genes, functional processes and associated potential promoter motifs

Authors

  • Carletha R. Blanding,

    1. Department of Biology and Marine Biology, University of North Carolina at Wilmington, 601 S. College, Wilmington, NC 28403, USA
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    • Present address: PPD, Inc., Wilmington, NC 28412, USA

  • Susan J. Simmons,

    1. Department of Mathematics and Statistics, University of North Carolina at Wilmington, 601 S. College, Wilmington, NC 28403, USA
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  • Paula Casati,

    1. Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
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    • Present address: CEFOBI, CONICET, Universidad Nacional de Rosario, Rosario, 2000 Santa Fe, Argentina

  • Virginia Walbot,

    1. Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
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  • Ann E. Stapleton

    Corresponding author
    1. Department of Biology and Marine Biology, University of North Carolina at Wilmington, 601 S. College, Wilmington, NC 28403, USA
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* Correspondence (fax (910) 962-4066; e-mail stapletona@uncw.edu)

Summary

Genetic gain in the yield of modern maize reflects increased stress tolerance. The manipulation of genes for deliberate alterations in tolerance relies on an understanding of the regulation and components of stress responses. Transcriptome analysis of an ultraviolet (UV) radiation time course with paired treatment and control measurements yielded groups of coordinately regulated genes and gene ontology processes. A comparison of the patterns of gene expression with patterns of morphological changes allowed the identification of physiologically relevant gene expression regulons. A set of genes significantly affected by UV radiation in maize leaves was selected by linear modelling plus order-restricted inference profile matches. This gene list was used to find upstream sequence motifs that predict the UV regulation of maize gene expression.

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