Present address: IASMA Research Centre, via E. Mach 1, 38010 S. Michele A/A, Trento, Italy
TILLMore, a resource for the discovery of chemically induced mutants in barley
Article first published online: 16 APR 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 6, Issue 5, pages 477–485, June 2008
How to Cite
Talamè, V., Bovina, R., Sanguineti, M. C., Tuberosa, R., Lundqvist, U. and Salvi, S. (2008), TILLMore, a resource for the discovery of chemically induced mutants in barley. Plant Biotechnology Journal, 6: 477–485. doi: 10.1111/j.1467-7652.2008.00341.x
- Issue published online: 16 APR 2008
- Article first published online: 16 APR 2008
- Received 10 January 2008; revised 18 February 2008; accepted 26 February 2008.
- chemical mutagenesis;
- forward genetics;
- Hordeum vulgare ;
- reverse genetics;
A sodium azide-mutagenized population of barley (cv. ‘Morex’) was developed and utilized to identify mutants at target genes using the ‘targeting induced local lesions in genomes’ (TILLING) procedure. Screening for mutations at four agronomically important genes (HvCO1, Rpg1, eIF4E and NR) identified a total of 22 new mutant alleles, equivalent to the extrapolated rate of one mutation every 374 kb. All mutations except one were G/C to A/T transitions and several (approximately 68%) implied a change in protein amino acid sequence and therefore a possible effect on phenotype. The high rate of mutation detected through TILLING is in keeping with the high frequency (32.7%) of variant phenotypes observed amongst the M3 families. Our results indicate the feasibility of using this resource for both reverse and forward genetics approaches to investigate gene function in barley and related crops.