• Open Access

TILLMore, a resource for the discovery of chemically induced mutants in barley

Authors

  • Valentina Talamè,

    1. Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
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  • Riccardo Bovina,

    1. Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
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  • Maria Corinna Sanguineti,

    1. Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
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  • Roberto Tuberosa,

    1. Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
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  • Udda Lundqvist,

    1. Nordic Genetic Resource Center, PO Box 41, SE-230 53 Alnarp, Sweden
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  • Silvio Salvi

    Corresponding author
    1. Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
      * Correspondence (fax +39-051-2096241; e-mail silvio.salvi@unibo.it)
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    • Present address: IASMA Research Centre, via E. Mach 1, 38010 S. Michele A/A, Trento, Italy


* Correspondence (fax +39-051-2096241; e-mail silvio.salvi@unibo.it)

Summary

A sodium azide-mutagenized population of barley (cv. ‘Morex’) was developed and utilized to identify mutants at target genes using the ‘targeting induced local lesions in genomes’ (TILLING) procedure. Screening for mutations at four agronomically important genes (HvCO1, Rpg1, eIF4E and NR) identified a total of 22 new mutant alleles, equivalent to the extrapolated rate of one mutation every 374 kb. All mutations except one were G/C to A/T transitions and several (approximately 68%) implied a change in protein amino acid sequence and therefore a possible effect on phenotype. The high rate of mutation detected through TILLING is in keeping with the high frequency (32.7%) of variant phenotypes observed amongst the M3 families. Our results indicate the feasibility of using this resource for both reverse and forward genetics approaches to investigate gene function in barley and related crops.

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