These authors contributed equally to this work
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Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect
Article first published online: 24 SEP 2008
DOI: 10.1111/j.1467-7652.2008.00374.x
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Additional Information
How to Cite
Usuda, K., Wada, Y., Ishimaru, Y., Kobayashi, T., Takahashi, M., Nakanishi, H., Nagato, Y., Mori, S. and Nishizawa, N. K. (2009), Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect. Plant Biotechnology Journal, 7: 87–95. doi: 10.1111/j.1467-7652.2008.00374.x
Publication History
- Issue published online: 12 DEC 2008
- Article first published online: 24 SEP 2008
- Received 3 March 2008; revised 18 August 2008; accepted 20 August 2008.
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Keywords:
- antihypertensive;
- cleistogamy;
- Cre/loxP DNA excision system;
- nicotianamine;
- rice;
- transgenic
Summary
Nicotianamine (NA), a metal chelator ubiquitous in higher plants, serves as an antihypertensive substance in humans. To engineer a novel antihypertensive rice that contains larger amounts of NA, the barley NA synthase gene, HvNAS1, was introduced into rice via Agrobacterium-mediated transformation. The introduced HvNAS1 was driven by pGluB-1, which induces strong gene expression in the endosperm of rice seeds. The NA content in transgenic rice seeds was up to fourfold greater than that in non-transgenic rice seeds. The Cre/loxP DNA excision (CLX) system was used to remove the selectable marker gene for antibiotic resistance. Furthermore, the transgenic rice was crossed with a cleistogamous mutant to prevent gene transfer via pollen dispersal. These two modifications may minimize public concern with regard to the use of this transgenic rice.