• Open Access

Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants

Authors

  • Louis-P. Vézina,

    Corresponding author
    1. Medicago Inc., 1020, route de l’Église, Bureau 600, Sainte-Foy, QC, Canada, G1V 3V9
      * Correspondence (fax +1 418 658 6699; e-mail vezinalp@medicago.com or fax +33 2 35 14 67 87; e-mail vgomord@crihan.fr)
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  • Loïc Faye,

    1. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
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  • Patrice Lerouge,

    1. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
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  • Marc-André D’Aoust,

    1. Medicago Inc., 1020, route de l’Église, Bureau 600, Sainte-Foy, QC, Canada, G1V 3V9
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  • Estelle Marquet-Blouin,

    1. Medicago Inc., 1020, route de l’Église, Bureau 600, Sainte-Foy, QC, Canada, G1V 3V9
    2. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
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  • Carole Burel,

    1. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
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  • Pierre-Olivier Lavoie,

    1. Medicago Inc., 1020, route de l’Église, Bureau 600, Sainte-Foy, QC, Canada, G1V 3V9
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  • Muriel Bardor,

    1. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
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  • Véronique Gomord

    Corresponding author
    1. CNRS UMR 6037, IFRMP 23, Université de Rouen, Faculté des Sciences, 76 821 Mont-Saint-Aignan, France
      * Correspondence (fax +1 418 658 6699; e-mail vezinalp@medicago.com or fax +33 2 35 14 67 87; e-mail vgomord@crihan.fr)
    Search for more papers by this author

* Correspondence (fax +1 418 658 6699; e-mail vezinalp@medicago.com or fax +33 2 35 14 67 87; e-mail vgomord@crihan.fr)

Summary

Plant-based transient expression is potentially the most rapid and cost-efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant-specific N-glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co-expression of an antibody, a suppressor of silencing and a chimaeric human β1,4-galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high-yield production of antibodies with human-like N-glycans and, more generally, provides solutions to many critical problems posed by the large-scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.

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