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Figure S1 Genetic analysis of progeny plantsobtained by self-pollination of four homozygous doubled haploid (DH) plants (DHnumbers 52, 53, 56, 61). The progeny were scored for the accumulation ofassembled antibody containing both immunoglobulin (Ig) chains by ProteinL/Protein A sandwich enzyme-linked immunosorbent assay (ELISA). BSA, bovineserum albumin; CHO2F5, antibody standard from Chinese hamster ovary(CHO) cells; Wt, non-transformed tobacco cv. SNN.

Figure S2 Genetic analysis of progeny plants obtainedby self-pollination of a hemizygous T2plant. T3 progeny werescored for the accumulation of assembled antibody containing bothimmunoglobulin (Ig) chains by Protein L/Protein A sandwich enzyme-linkedimmunosorbent assay (ELISA). BSA, bovine serum albumin; CHO2F5,antibody standard from Chinese hamster ovary (CHO) cells; Wt, non-transformedtobacco cv. SNN.

Table S1 Transgene segregation in the T4 generation derived by self-pollination ofhemizygous T3 plants expressingan assembled monoclonal antibody (mAb). The T3 plant numbers correspond to those in Figure S2.

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