These authors contributed equally to this work.
Insertion site-based polymorphism markers open new perspectives for genome saturation and marker-assisted selection in wheat
Article first published online: 11 JAN 2010
© 2010 INRA. Journal compilation © 2010 Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 8, Issue 2, pages 196–210, February 2010
How to Cite
Paux, E., Faure, S., Choulet, F., Roger, D., Gauthier, V., Martinant, J.-P., Sourdille, P., Balfourier, F., Le Paslier, M.-C., Chauveau, A., Cakir, M., Gandon, B. and Feuillet, C. (2010), Insertion site-based polymorphism markers open new perspectives for genome saturation and marker-assisted selection in wheat. Plant Biotechnology Journal, 8: 196–210. doi: 10.1111/j.1467-7652.2009.00477.x
- Issue published online: 11 JAN 2010
- Article first published online: 11 JAN 2010
- Received 17 July 2009; revised 15 October 2009; accepted 15 October 2009.
- Insertion site-based polymorphism;
- molecular marker;
- marker-assisted selection;
In wheat, the deployment of marker-assisted selection has long been hampered by the lack of markers compatible with high-throughput cost-effective genotyping techniques. Recently, insertion site-based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high-throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP-derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost-effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker-assisted selection.