Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement
Article first published online: 17 MAY 2010
© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 1, pages 38–49, January 2011
How to Cite
Fukuzawa, N., Ishihara, T., Itchoda, N., Tabayashi, N., Kataoka, C., Masuta, C. and Matsumura, T. (2011), Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement. Plant Biotechnology Journal, 9: 38–49. doi: 10.1111/j.1467-7652.2010.00529.x
- Issue published online: 6 DEC 2010
- Article first published online: 17 MAY 2010
- Received 30 September 2009;revised 5 March 2010;accepted 15 March 2010.
- plant virus vector;
- recombinant protein
A plant viral vector has the potential to efficiently produce recombinant proteins at a low cost in a short period. Although recombinant proteins can be also produced by transgenic plants, a plant viral vector, if available, may be more convenient when urgent scale-up in production is needed. However, it is difficult to use a viral vector in open fields because of the risk of escape to the environment. In this study, we constructed a novel viral vector system using a movement-defective Cucumber mosaic virus (CMV) vector, which is theoretically localized in the inoculated cells but infects systemically only with the aid of the transgenic helper plant that complements viral movement, diminishing the risk of viral proliferation. Interestingly, the helper plant systemically infected with the vector gave strong cross-protection against challenge inoculation with wild-type CMVs. Using CMV strains belonging to two discrete CMV groups (subgroups I and II), we also improved the system to prevent recombination between the vector and the transgene transcript in the helper plant. We here demonstrate the expression of an anti-dioxin single chain variable fragment (DxscFv) and interleukin-1 receptor antagonist (IL1-Ra) in Nicotiana benthamiana by this viral vector confinement system, which is applicable for many useful high-quality recombinant proteins.