Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement
Article first published online: 17 MAY 2010
© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 1, pages 38–49, January 2011
How to Cite
Fukuzawa, N., Ishihara, T., Itchoda, N., Tabayashi, N., Kataoka, C., Masuta, C. and Matsumura, T. (2011), Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement. Plant Biotechnology Journal, 9: 38–49. doi: 10.1111/j.1467-7652.2010.00529.x
- Issue published online: 6 DEC 2010
- Article first published online: 17 MAY 2010
- Received 30 September 2009;revised 5 March 2010;accepted 15 March 2010.
Figure S1 Analysis of DxscFv expressed by VCS in helper plants.
Figure S2 Schematic representation of the serial passage experiments under extreme conditions to induce recombination between the m2∆3a or Y∆3a and PVX-Y3a.
Table S1 Expression levels of the CMV-Y3a protein in transgenic Nicotiana Benthamiana plants (T0).
Table S2 Summary of recombination between Δ3a constructs (YΔ3a and m2Δ3a) and PVX-Y3a in the mixed infection in N. benthamiana by serial passages.
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