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Figure S1 Analysis of DxscFv expressed by VCS in helper plants.

Figure S2 Schematic representation of the serial passage experiments under extreme conditions to induce recombination between the m2∆3a or Y∆3a and PVX-Y3a.

Table S1 Expression levels of the CMV-Y3a protein in transgenic Nicotiana Benthamiana plants (T0).

Table S2 Summary of recombination between Δ3a constructs (YΔ3a and m2Δ3a) and PVX-Y3a in the mixed infection in N.  benthamiana by serial passages.

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