Current address: Department of Applied Biology, Gyeongsang National University, Jinju 660-701, Korea.
A novel light-dependent selection marker system in plants
Article first published online: 23 AUG 2010
© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 3, pages 348–358, April 2011
How to Cite
Koh, S., Kim, H., Kim, J., Goo, E., Kim, Y.-J., Choi, O., Jwa, N.-S., Ma, J., Nagamatsu, T., Moon, J. S. and Hwang, I. (2011), A novel light-dependent selection marker system in plants. Plant Biotechnology Journal, 9: 348–358. doi: 10.1111/j.1467-7652.2010.00557.x
tflA (GenBank accession number GQ921834).
- Issue published online: 6 MAR 2011
- Article first published online: 23 AUG 2010
- Received 14 February 2010; revised 21 June 2010; accepted 24 June 2010.
- selection marker system;
Photosensitizers are common in nature and play diverse roles as defense compounds and pathogenicity determinants and as important molecules in many biological processes. Toxoflavin, a photosensitizer produced by Burkholderia glumae, has been implicated as an essential virulence factor causing bacterial rice grain rot. Toxoflavin produces superoxide and H2O2 during redox cycles under oxygen and light, and these reactive oxygen species cause phytotoxic effects. To utilize toxoflavin as a selection agent in plant transformation, we identified a gene, tflA, which encodes a toxoflavin-degrading enzyme in the Paenibacillus polymyxa JH2 strain. TflA was estimated as 24.56 kDa in size based on the amino acid sequence and is similar to a ring-cleavage extradiol dioxygenase in the Exiguobacterium sp. 255-15; however, unlike other extradiol dioxygenases, Mn2+and dithiothreitol were required for toxoflavin degradation by TflA. Here, our results suggested toxoflavin is a photosensitizer and its degradation by TflA serves as a light-dependent selection marker system in diverse plant species. We examined the efficiencies of two different plant selection systems, toxoflavin/tflA and hygromycin/hygromycin phosphotransferase (hpt) in both rice and Arabidopsis. The toxoflavin/tflA selection was more remarkable than hygromycin/hpt selection in the high-density screening of transgenic Arabidopsis seeds. Based on these results, we propose the toxoflavin/tflA selection system, which is based on the degradation of the photosensitizer, provides a new robust nonantibiotic selection marker system for diverse plants.