Present address: Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Qld 4072 Australia.
Chloroplast genome sequences from total DNA for plant identification
Article first published online: 26 AUG 2010
© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 3, pages 328–333, April 2011
How to Cite
Nock, C. J., Waters, D. L.E., Edwards, M. A., Bowen, S. G., Rice, N., Cordeiro, G. M. and Henry, R. J. (2011), Chloroplast genome sequences from total DNA for plant identification. Plant Biotechnology Journal, 9: 328–333. doi: 10.1111/j.1467-7652.2010.00558.x
- Issue published online: 6 MAR 2011
- Article first published online: 26 AUG 2010
- Received 3 March 2010;revised 6 June 2010;accepted 8 June 2010.
- DNA barcode;
- massively parallel sequencing
Chloroplast DNA sequence data are a versatile tool for plant identification or barcoding and establishing genetic relationships among plant species. Different chloroplast loci have been utilized for use at close and distant evolutionary distances in plants, and no single locus has been identified that can distinguish between all plant species. Advances in DNA sequencing technology are providing new cost-effective options for genome comparisons on a much larger scale. Universal PCR amplification of chloroplast sequences or isolation of pure chloroplast fractions, however, are non-trivial. We now propose the analysis of chloroplast genome sequences from massively parallel sequencing (MPS) of total DNA as a simple and cost-effective option for plant barcoding, and analysis of plant relationships to guide gene discovery for biotechnology. We present chloroplast genome sequences of five grass species derived from MPS of total DNA. These data accurately established the phylogenetic relationships between the species, correcting an apparent error in the published rice sequence. The chloroplast genome may be the elusive single-locus DNA barcode for plants.