Phospholipid and triacylglycerol profiles modified by PLD suppression in soybean seed
Article first published online: 26 AUG 2010
© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 3, pages 359–372, April 2011
How to Cite
Lee, J., Welti, R., Schapaugh, W. T. and Trick, H. N. (2011), Phospholipid and triacylglycerol profiles modified by PLD suppression in soybean seed. Plant Biotechnology Journal, 9: 359–372. doi: 10.1111/j.1467-7652.2010.00562.x
- Issue published online: 6 MAR 2011
- Article first published online: 26 AUG 2010
- Received 2 April 2010;revised 2 June 2010;accepted 29 June 2010.
- genetic transformation;
- phospholipase D;
- soybean lecithin
Phospholipase D (PLD) is capable of hydrolyzing membrane phospholipids, producing phosphatidic acid. To alter phospholipid profiles in soybean seed, we attenuated PLD enzyme activity by an RNA interference construct using the partial sequence from a soybean PLDα gene. Two transgenic soybean lines were established by particle inflow gun (PIG) bombardment by co-bombarding with pSPLDi and pHG1 vectors. The lines were evaluated for the presence and expression of transgenes thoroughly through the T4 generation. PLD-suppressed soybean lines were characterized by decreased PLDα enzyme activity and decreased PLDα protein both during seed development and in mature seeds. There was no change in total phospholipid amount; however, the PLD-attenuated transgenic soybean seed had higher levels of di18 : 2 (dilinoleoyl)-phosphatidylcholine (PC) and -phosphatidylethanolamine (PE) in seeds than the non-transgenic lines. The increased polyunsaturation was at the expense of PC and PE species containing monounsaturated or saturated fatty acids. In addition to increased unsaturation in the phospholipids, there was a decrease in unsaturation of the triacylglycerol (TAG) fraction of the soybean seeds. Considering recent evidence for the notion that desaturation of fatty acids occurs in the PC fraction and that the PC DAG (diacylglycerol) TAG pathway is the major route of TAG biosynthesis in developing soybean seed, the current data suggest that PLDα suppression slows the conversion of PC to TAG. This would be consistent with PLD playing a positive role in that conversion. The data indicate that soybean PLD attenuation is a potentially useful approach to altering properties of edible and industrial soybean lecithin.