Present address: VTT Technical Research Centre of Finland, Tietotie 2, 02040 VTT, Finland.
Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
Article first published online: 7 OCT 2010
© 2010 Crown in the right of Canada. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 4, pages 434–444, May 2011
How to Cite
Conley, A. J., Zhu, H., Le, L. C., Jevnikar, A. M., Lee, B. H., Brandle, J. E. and Menassa, R. (2011), Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis. Plant Biotechnology Journal, 9: 434–444. doi: 10.1111/j.1467-7652.2010.00563.x
- Issue published online: 17 APR 2011
- Article first published online: 7 OCT 2010
- Received 28 January 2010; revised 27 May 2010; accepted 8 July 2010.
- molecular farming;
- recombinant protein production;
- transgenic plants;
- transient expression;
- Pseudomonas aeruginosa
Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin-10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable α-amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.