• Alvarez, M.L., Pinyerd HL, E.T. and Cardineau, G.A. (2008) P19-dependent and P19-independent reversion of F1-V gene silencing in tomato. Plant Mol. Biol., 68, 6179.
  • Buchacher, A., Predl, R., Strutzenberger, K., Steinfellner, W., Trkola, A., Purtscher, M., Gruber, G., Tauer, C., Steindl, F., Jungbauer, A. and Katringer, H. (1994) Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization. AIDS Res. Hum. Retroviruses, 10, 359369.
  • Cañizares, M.C., Nicholson, L. and Lomonossoff, G.P. (2005) Use of viral vectors for vaccine production in plants. Immunol. Cell Biol., 83, 263270.
    Direct Link:
  • Chapman, E., Prokhnevsky, A.I., Gopinath, K., Dolja, V.V. and Carrington, J.C. (2004) Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step. Genes Dev., 18, 11791186.
  • Chu, M., Desvoyes, B., Turina, M., Noad, R. and Scholthof, H.B. (2000) Genetic dissection of Tomato bushy stunt virus p19-protein-mediated host-dependent symptom induction and systemic invasion. Virology, 266, 7987.
  • Dunoyer, P., Lecellier, C.H., Parizotto, E.A., Himber, C. and Voinnet, O. (2004) Probing the microRNA and small interfering RNA pathways with virus-encoded suppressors of RNA silencing. Plant Cell, 16, 12351250.
  • Goldberg, K.B., Kiernan, J. and Shepherd, R.J. (1991) A disease syndrome associated with expression of gene-VI of Caulimoviruses may be a nonhost reaction. Mol. Plant-Microbe Interact, 4, 182189.
  • Horsch, R.B. and Klee, H.J. (1986) Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: role of T-DNA borders in the transfer process. Proc. Natl. Acad. Sci. USA, 83, 44284432.
  • Johansen, L.K. and Carrington, J.C. (2001) Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol., 126, 930938.
  • Lakatos, L., Szittya, G., Silhavy, D. and Burgyan, J. (2004) Molecular mechanism of RNA silencing suppression mediated by the p19 protein of tombusviruses. EMBO J., 23, 876884.
  • Mallory, A.C., Parks, G., Endres, M.W., Baulcombe, D., Bowman, L.H., Pruss, G.J. and Vance, V.B. (2002) The amplicon-plus system for high-level expression of transgenes in plants. Nat. Biotechnol., 20, 622625.
  • Omarov, R., Sparks, K., Smith, L., Zindovic, J. and Scholthof, H.B. (2006) Biological relevance of a stable biochemical interaction between the tombusvirus-encoded P19 and short interfering RNAs. J. Virol., 80, 30003008.
  • Omarov, R.T., Ciomperlik, J.J. and Scholthof, H.B. (2007) RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex. Proc. Natl. Acad. Sci. USA, 104, 17141719.
  • Pantaleo, V., Szittya, G. and Burgyan, J. (2007) Molecular basis of viral RNA targeting by viral small interfering RNA-programmed RISC. J. Virol., 81, 37973806.
  • Papp, I., Mette, M.F., Aufsatz, W., Daxinger, L., Schauer, S.E., Ray, A., van der Winden, J., Matzke, M. and Matzke, A.J.M. (2003) Evidence for nuclear processing of plant microRNA and short-interfering RNA precursors. Plant Physiol., 132, 13821390.
  • Park, J.-W., Faure-Rabasse, S., Robinson, M.A., Desvoyes, B. and Scholthof, H.B. (2004) The multifunctional plant viral suppressor of gene silencing P19 interacts with itself and an RNA binding host protein. Virology, 323, 4958.
  • Qiu, W. and Scholthof, H.B. (2007) Tomato bushy stunt virus derived gene vectors. Curr Prot Microbiol 7:16I.14.1116I.14.16.
  • Qiu, W.P., Park, J.-W. and Scholthof, H.B. (2002) Tombusvirus P19-mediated suppression of virus induced gene silencing is controlled by genetic and dosage features that influence pathogenicity. Mol. Plant-Microbe Interact., 15, 269280.
  • Richards, H.A., Halfhill, M.D., Millwood, R.J. and Stewart Jr, C.N. (2003) Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Rep., 22, 117121.
  • Sainsbury, F. and Lomonossoff, G.P. (2008) Extremely high-level and rapid transient protein production in plants without the use of viral replication. Plant Physiol., 148, 12121218.
  • Sainsbury, F., Thuenemann, E.C. and Lomonossoff, G.P. (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol. J., 7, 682693.
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor, N.Y.:Cold Spring Harbor Laboratory.
  • Scholthof, H.B. (2006) The Tombusvirus-encoded P19: from irrelevance to elegance. Nat. Rev. Microbiol., 4, 405411.
  • Scholthof, H.B. (2007) Heterologous expression of viral RNA interference suppressors: RISC management. Plant Physiol., 145, 11101117.
  • Scholthof, H.B., Morris, T.J. and Jackson, A.O. (1993) The capsid protein gene of Tomato bushy stunt virus is dispensable for systemic movement and can be replaced for localized expression of foreign genes. Mol. Plant-Microbe Interact., 6, 309322.
  • Scholthof, H.B., Scholthof, K.-B.G. and Jackson, A.O. (1995) Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell, 7, 11571172.
  • Scholthof, K.-B.G., Mirkov, T.E. and Scholthof, H.B. (2002) Plant viral gene vectors: biotechnology applications in agriculture and medicine. Genet. Eng. Principles Methods, 24, 6786.
  • Siddiqui, S.A., Sarmiento, C., Truve, E., Lehto, H. and Lehto, K. (2008) Phenotypes and functional effects caused by various viral RNA silencing suppressors in transgenic Nicotiana benthamiana and N. tabacum. Mol. Plant-Microbe Interact., 21, 178187.
  • Silhavy, D., Molnar, A., Lucioli, A., Szittya, G., Hornyik, C., Tavazza, M. and Burgyan, J. (2002) A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs. EMBO J., 21, 30703080.
  • Vargason, J.M., Szittya, G., Burgyan, J. and Tanaka- Hall, T.M. (2003) Size selective recognition of siRNA by an RNA silencing suppressor. Cell, 115, 799811.
  • Voinnet, O. (2005) Induction and suppression of RNA silencing: insights from viral infections. Nat. Rev. Genetics, 6, 206220.
  • Voinnet, O., Vain, P., Angell, S. and Baulcombe, D.C. (1998) Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell, 95, 177187.
  • Voinnet, O., Rivas, S., Mestre, P. and Baulcombe, D. (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J., 33, 949956.
  • Wroblewski, T., Tomczak, A. and Michelmore, R. (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol. J., 3, 259273.
  • Ye, K., Malinina, L. and Patel, D. (2003) Recognition of small interfering RNA by a viral suppressor of RNA silencing. Nature, 426, 874878.